Optogenetic control of Cofilin and αTAT in living cells using Z-lock

Here we introduce Z-lock, an optogenetic approach for reversible, light-controlled steric inhibition of protein active sites. The LOV domain and Zdk, a small protein that binds LOV selectively in the dark, are appended to the protein of interest where they sterically block the active site. Irradiati...

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Detalles Bibliográficos
Autores principales: Stone, Orrin J., Pankow, Neha, Liu, Bei, Sharma, Ved P., Eddy, Robert J., Wang, Hui, Putz, Andrew T., Teets, Frank D, Kuhlman, Brian, Condeelis, John S., Hahn, Klaus M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873228/
https://www.ncbi.nlm.nih.gov/pubmed/31740825
http://dx.doi.org/10.1038/s41589-019-0405-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873228/
https://www.ncbi.nlm.nih.gov/pubmed/31740825
http://dx.doi.org/10.1038/s41589-019-0405-4

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