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Optogenetic control of Cofilin and αTAT in living cells using Z-lock
Here we introduce Z-lock, an optogenetic approach for reversible, light-controlled steric inhibition of protein active sites. The LOV domain and Zdk, a small protein that binds LOV selectively in the dark, are appended to the protein of interest where they sterically block the active site. Irradiati...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873228/ https://www.ncbi.nlm.nih.gov/pubmed/31740825 http://dx.doi.org/10.1038/s41589-019-0405-4 |