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Age-associated changes in human CD4(+) T cells point to mitochondrial dysfunction consequent to impaired autophagy

To gain understanding on the mechanisms that drive immunosenescence in humans, we examined CD4(+) T cells obtained from younger (20-39 years-old) and older (70+ years-old) healthy participants of the Baltimore Longitudinal Study on Aging (BLSA). We found that mitochondrial proteins involved in the e...

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Detalles Bibliográficos
Autores principales: Bektas, Arsun, Schurman, Shepherd H., Gonzalez-Freire, Marta, Dunn, Christopher A., Singh, Amit K., Macian, Fernando, Cuervo, Ana Maria, Sen, Ranjan, Ferrucci, Luigi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874450/
https://www.ncbi.nlm.nih.gov/pubmed/31707363
http://dx.doi.org/10.18632/aging.102438
Descripción
Sumario:To gain understanding on the mechanisms that drive immunosenescence in humans, we examined CD4(+) T cells obtained from younger (20-39 years-old) and older (70+ years-old) healthy participants of the Baltimore Longitudinal Study on Aging (BLSA). We found that mitochondrial proteins involved in the electron transport chain were overrepresented in cells from older participants, with prevalent dysregulation of oxidative phosphorylation and energy metabolism molecular pathways. Surprisingly, gene transcripts coding for mitochondrial proteins pertaining to oxidative phosphorylation and electron transport chain pathways were underrepresented in older individuals. Paralleling the observed decrease in gene expression, mitochondrial respiration was impaired in CD4(+) T cells from older subjects. Though mitochondrial number in both naïve and memory cells visualized with electron microcopy was similar in older versus younger participants, there were a significantly higher number of autophagosomes, many of them containing undegraded mitochondria, in older individuals. The presence of mitochondria inside the accumulated autophagic compartments in CD4(+) T cells from older individuals was confirmed by immunofluorescence. These findings suggest that older age is associated with persistence of dysfunctional mitochondria in CD4(+) T lymphocytes caused by defective mitochondrial turnover by autophagy, which may trigger chronic inflammation and contribute to the impairment of immune defense in older persons.