Improved strategy for isoleucine (1)H/(13)C methyl labeling in Pichia pastoris

Site specific methyl labeling combined with methyl TROSY offers a powerful NMR approach to study structure and dynamics of proteins and protein complexes of high molecular weight. Robust and cost-effective methods have been developed for site specific protein (1)H/(13)C methyl labeling in an otherwi...

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Detalles Bibliográficos
Autores principales: Ali, Rustam, Clark, Lindsay D., Zahm, Jacob A., Lemoff, Andrew, Ramesh, Karthik, Rosenbaum, Daniel M., Rosen, Michael K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6875547/
https://www.ncbi.nlm.nih.gov/pubmed/31541396
http://dx.doi.org/10.1007/s10858-019-00281-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6875547/
https://www.ncbi.nlm.nih.gov/pubmed/31541396
http://dx.doi.org/10.1007/s10858-019-00281-1

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