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Improved strategy for isoleucine (1)H/(13)C methyl labeling in Pichia pastoris
Site specific methyl labeling combined with methyl TROSY offers a powerful NMR approach to study structure and dynamics of proteins and protein complexes of high molecular weight. Robust and cost-effective methods have been developed for site specific protein (1)H/(13)C methyl labeling in an otherwi...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer Netherlands
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6875547/ https://www.ncbi.nlm.nih.gov/pubmed/31541396 http://dx.doi.org/10.1007/s10858-019-00281-1 |