Differential gene expression in chronic wasting disease‐positive white‐tailed deer (Odocoileus virginianus)

Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) that affects cervid species throughout North America. We evaluated gene expression in white‐tailed deer collected by Illinois Department of Natural Resource wildlife managers during annual population reduction (e.g., sh...

Descripción completa

Detalles Bibliográficos
Autores principales: Trone‐Launer, Emma K., Wang, Jun, Lu, Guoqing, Mateus‐Pinilla, Nohra E., Zick, Paige R., Lamer, James T., Shelton, Paul A., Jacques, Christopher N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6875659/
https://www.ncbi.nlm.nih.gov/pubmed/31788200
http://dx.doi.org/10.1002/ece3.5724
_version_ 1783473070925676544
author Trone‐Launer, Emma K.
Wang, Jun
Lu, Guoqing
Mateus‐Pinilla, Nohra E.
Zick, Paige R.
Lamer, James T.
Shelton, Paul A.
Jacques, Christopher N.
author_facet Trone‐Launer, Emma K.
Wang, Jun
Lu, Guoqing
Mateus‐Pinilla, Nohra E.
Zick, Paige R.
Lamer, James T.
Shelton, Paul A.
Jacques, Christopher N.
author_sort Trone‐Launer, Emma K.
collection PubMed
description Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) that affects cervid species throughout North America. We evaluated gene expression in white‐tailed deer collected by Illinois Department of Natural Resource wildlife managers during annual population reduction (e.g., sharpshooting) and disease monitoring efforts throughout the CWD‐endemic area of northcentral Illinois. We conducted comparative transcriptomic analysis of liver and retropharyngeal lymph node tissue samples between CWD‐positive (n = 5) and CWD‐not detected (n = 5) deer. A total of 74,479 transcripts were assembled, and 51,661 (69.36%) transcripts were found to have matched proteins in NCBI‐NR and UniProt. Our analysis of functional categories showed 40,308 transcripts were assigned to at least one Gene Ontology term and 37,853 transcripts were involved in at least one pathway. We identified a total of 59 differentially expressed genes (DEGs) in CWD‐positive deer, of which 36 and 23 were associated with liver and retropharyngeal lymph node tissues, respectively. Functions of DEGs lend support to previous relationships between misfolded PrP and cellular membranes (e.g., STXBP5), and internal cellular components. We identified several genes that suggest a link between CWD and retroviruses and identified the gene ADIPOQ that acts as a tumor necrosis factor (TNF) antagonist. This gene may lead to reduced production of TNF and impact disease progression and clinical symptoms associated with CWD (i.e., wasting syndrome). Use of candidate genes identified in this study suggests the activation of endogenous processes in CWD‐positive deer, which in turn may enable earlier detection of the disease.
format Online
Article
Text
id pubmed-6875659
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-68756592019-11-29 Differential gene expression in chronic wasting disease‐positive white‐tailed deer (Odocoileus virginianus) Trone‐Launer, Emma K. Wang, Jun Lu, Guoqing Mateus‐Pinilla, Nohra E. Zick, Paige R. Lamer, James T. Shelton, Paul A. Jacques, Christopher N. Ecol Evol Original Research Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) that affects cervid species throughout North America. We evaluated gene expression in white‐tailed deer collected by Illinois Department of Natural Resource wildlife managers during annual population reduction (e.g., sharpshooting) and disease monitoring efforts throughout the CWD‐endemic area of northcentral Illinois. We conducted comparative transcriptomic analysis of liver and retropharyngeal lymph node tissue samples between CWD‐positive (n = 5) and CWD‐not detected (n = 5) deer. A total of 74,479 transcripts were assembled, and 51,661 (69.36%) transcripts were found to have matched proteins in NCBI‐NR and UniProt. Our analysis of functional categories showed 40,308 transcripts were assigned to at least one Gene Ontology term and 37,853 transcripts were involved in at least one pathway. We identified a total of 59 differentially expressed genes (DEGs) in CWD‐positive deer, of which 36 and 23 were associated with liver and retropharyngeal lymph node tissues, respectively. Functions of DEGs lend support to previous relationships between misfolded PrP and cellular membranes (e.g., STXBP5), and internal cellular components. We identified several genes that suggest a link between CWD and retroviruses and identified the gene ADIPOQ that acts as a tumor necrosis factor (TNF) antagonist. This gene may lead to reduced production of TNF and impact disease progression and clinical symptoms associated with CWD (i.e., wasting syndrome). Use of candidate genes identified in this study suggests the activation of endogenous processes in CWD‐positive deer, which in turn may enable earlier detection of the disease. John Wiley and Sons Inc. 2019-10-30 /pmc/articles/PMC6875659/ /pubmed/31788200 http://dx.doi.org/10.1002/ece3.5724 Text en © 2019 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Trone‐Launer, Emma K.
Wang, Jun
Lu, Guoqing
Mateus‐Pinilla, Nohra E.
Zick, Paige R.
Lamer, James T.
Shelton, Paul A.
Jacques, Christopher N.
Differential gene expression in chronic wasting disease‐positive white‐tailed deer (Odocoileus virginianus)
title Differential gene expression in chronic wasting disease‐positive white‐tailed deer (Odocoileus virginianus)
title_full Differential gene expression in chronic wasting disease‐positive white‐tailed deer (Odocoileus virginianus)
title_fullStr Differential gene expression in chronic wasting disease‐positive white‐tailed deer (Odocoileus virginianus)
title_full_unstemmed Differential gene expression in chronic wasting disease‐positive white‐tailed deer (Odocoileus virginianus)
title_short Differential gene expression in chronic wasting disease‐positive white‐tailed deer (Odocoileus virginianus)
title_sort differential gene expression in chronic wasting disease‐positive white‐tailed deer (odocoileus virginianus)
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6875659/
https://www.ncbi.nlm.nih.gov/pubmed/31788200
http://dx.doi.org/10.1002/ece3.5724
work_keys_str_mv AT tronelauneremmak differentialgeneexpressioninchronicwastingdiseasepositivewhitetaileddeerodocoileusvirginianus
AT wangjun differentialgeneexpressioninchronicwastingdiseasepositivewhitetaileddeerodocoileusvirginianus
AT luguoqing differentialgeneexpressioninchronicwastingdiseasepositivewhitetaileddeerodocoileusvirginianus
AT mateuspinillanohrae differentialgeneexpressioninchronicwastingdiseasepositivewhitetaileddeerodocoileusvirginianus
AT zickpaiger differentialgeneexpressioninchronicwastingdiseasepositivewhitetaileddeerodocoileusvirginianus
AT lamerjamest differentialgeneexpressioninchronicwastingdiseasepositivewhitetaileddeerodocoileusvirginianus
AT sheltonpaula differentialgeneexpressioninchronicwastingdiseasepositivewhitetaileddeerodocoileusvirginianus
AT jacqueschristophern differentialgeneexpressioninchronicwastingdiseasepositivewhitetaileddeerodocoileusvirginianus