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Optimization of The Cell Aggregates Method for Isolation and Purification of Human Granulosa Cells from Follicular Fluid

BACKGROUND: Aspirated ovarian follicular fluids (FF) contain luteal granulosa cells (LGCs) and other contaminating cell types. Several strategies, such as the antibody binding methods, the flask method, the cell strainer and positive selection of granulosa aggregates after density gradient (DG) cent...

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Detalles Bibliográficos
Autores principales: Raad, Georges, Bazzi, Marwa, Tanios, Judy, Mourad, Youmna, Azouri, Joan, Azouri, Joseph, Fakih, Chadi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6875859/
https://www.ncbi.nlm.nih.gov/pubmed/31710196
http://dx.doi.org/10.22074/ijfs.2020.5790