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Optimization of The Cell Aggregates Method for Isolation and Purification of Human Granulosa Cells from Follicular Fluid
BACKGROUND: Aspirated ovarian follicular fluids (FF) contain luteal granulosa cells (LGCs) and other contaminating cell types. Several strategies, such as the antibody binding methods, the flask method, the cell strainer and positive selection of granulosa aggregates after density gradient (DG) cent...
Autores principales: | Raad, Georges, Bazzi, Marwa, Tanios, Judy, Mourad, Youmna, Azouri, Joan, Azouri, Joseph, Fakih, Chadi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6875859/ https://www.ncbi.nlm.nih.gov/pubmed/31710196 http://dx.doi.org/10.22074/ijfs.2020.5790 |
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