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Patterns of PCR Amplification Artifacts of the Fungal Barcode Marker in a Hybrid Mushroom
The polymerase chain reaction (PCR) is widely used in modern biology and medicine. However, PCR artifacts can complicate the interpretation of PCR-based results. The internal transcribed spacer (ITS) region of the ribosomal RNA gene cluster is the consensus fungal barcode marker and suspected PCR ar...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6877668/ https://www.ncbi.nlm.nih.gov/pubmed/31803173 http://dx.doi.org/10.3389/fmicb.2019.02686 |