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Patterns of PCR Amplification Artifacts of the Fungal Barcode Marker in a Hybrid Mushroom

The polymerase chain reaction (PCR) is widely used in modern biology and medicine. However, PCR artifacts can complicate the interpretation of PCR-based results. The internal transcribed spacer (ITS) region of the ribosomal RNA gene cluster is the consensus fungal barcode marker and suspected PCR ar...

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Detalles Bibliográficos
Autores principales: Zhou, Jun-Liang, Xu, Jianping, Jiao, An-Guo, Yang, Li, Chen, Jie, Callac, Philippe, Liu, Yu, Wang, Shou-Xian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6877668/
https://www.ncbi.nlm.nih.gov/pubmed/31803173
http://dx.doi.org/10.3389/fmicb.2019.02686