A single plasmid based CRISPR interference in Synechocystis 6803 – A proof of concept

We developed a simple method to apply CRISPR interference by modifying an existing plasmid pCRISPathBrick containing the native S. pyogenes CRISPR assembly for Synechocystis PCC6803 and named it pCRPB1010. The technique presented here using deadCas9 is easier to implement for gene silencing in Synec...

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Bibliografische gegevens
Hoofdauteurs: Kirtania, Prithwiraj, Hódi, Barbara, Mallick, Ivy, Vass, István Zoltan, Fehér, Tamás, Vass, Imre, Kós, Peter B.
Formaat: Online Artikel Texto
Taal:English
Gepubliceerd in: Public Library of Science 2019
Onderwerpen:
Research Article
Online toegang:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879144/
https://www.ncbi.nlm.nih.gov/pubmed/31770415
http://dx.doi.org/10.1371/journal.pone.0225375

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879144/
https://www.ncbi.nlm.nih.gov/pubmed/31770415
http://dx.doi.org/10.1371/journal.pone.0225375

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