A single plasmid based CRISPR interference in Synechocystis 6803 – A proof of concept

We developed a simple method to apply CRISPR interference by modifying an existing plasmid pCRISPathBrick containing the native S. pyogenes CRISPR assembly for Synechocystis PCC6803 and named it pCRPB1010. The technique presented here using deadCas9 is easier to implement for gene silencing in Synec...

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Detalles Bibliográficos
Autores principales: Kirtania, Prithwiraj, Hódi, Barbara, Mallick, Ivy, Vass, István Zoltan, Fehér, Tamás, Vass, Imre, Kós, Peter B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879144/
https://www.ncbi.nlm.nih.gov/pubmed/31770415
http://dx.doi.org/10.1371/journal.pone.0225375

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