An Inexpensive and Accurate Reverse Transcription-PCR–Melting Temperature Analysis Assay for Real-Time Influenza Virus B Lineage Discrimination

In this work, we describe a SYBR-Green one-step reverse transcription-PCR protocol coupled with a melting temperature analysis (RT-PCR-T(m)), which allows the discrimination of influenza B lineages Yamagata and Victoria. The assay is performed using a regular real-time thermocycler and is based on d...

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Detalles Bibliográficos
Autores principales: Motta, Fernando Couto, Born, Priscila Silva, Resende, Paola Cristina, Brown, David, Siqueira, Marilda Mendonça
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2019
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879292/
https://www.ncbi.nlm.nih.gov/pubmed/31554673
http://dx.doi.org/10.1128/JCM.00602-19

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879292/
https://www.ncbi.nlm.nih.gov/pubmed/31554673
http://dx.doi.org/10.1128/JCM.00602-19

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