Cargando…

An Inexpensive and Accurate Reverse Transcription-PCR–Melting Temperature Analysis Assay for Real-Time Influenza Virus B Lineage Discrimination

In this work, we describe a SYBR-Green one-step reverse transcription-PCR protocol coupled with a melting temperature analysis (RT-PCR-T(m)), which allows the discrimination of influenza B lineages Yamagata and Victoria. The assay is performed using a regular real-time thermocycler and is based on d...

Descripción completa

Detalles Bibliográficos
Autores principales:	Motta, Fernando Couto, Born, Priscila Silva, Resende, Paola Cristina, Brown, David, Siqueira, Marilda Mendonça
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2019
Materias:	Virology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879292/ https://www.ncbi.nlm.nih.gov/pubmed/31554673 http://dx.doi.org/10.1128/JCM.00602-19

Ejemplares similares

A Simple Reverse Transcriptase PCR Melting-Temperature Assay To Rapidly Screen for Widely Circulating SARS-CoV-2 Variants
por: Banada, Padmapriya, et al.
Publicado: (2021)

Highly Sensitive Lineage Discrimination of SARS-CoV-2 Variants through Allele-Specific Probe PCR
por: Ratcliff, Jeremy, et al.
Publicado: (2022)

Development and Evaluation of an Enterovirus D68 Real-Time Reverse Transcriptase PCR Assay
por: Wylie, Todd N., et al.
Publicado: (2015)

Development and evaluation of reverse transcription-insulated isothermal PCR assay to detect duck hepatitis A virus type A in liver samples using the POCKIT(TM) system
por: REN, Yupeng, et al.
Publicado: (2019)

Clinical Evaluation of Self-Collected Saliva by Quantitative Reverse Transcription-PCR (RT-qPCR), Direct RT-qPCR, Reverse Transcription–Loop-Mediated Isothermal Amplification, and a Rapid Antigen Test To Diagnose COVID-19
por: Nagura-Ikeda, Mayu, et al.
Publicado: (2020)

Comparison of Two Commercial Molecular Tests and a Laboratory-Developed Modification of the CDC 2019-nCoV Reverse Transcriptase PCR Assay for the Detection of SARS-CoV-2
por: Moore, Nicholas M., et al.
Publicado: (2020)

A Recurrent Mutation at Position 26340 of SARS-CoV-2 Is Associated with Failure of the E Gene Quantitative Reverse Transcription-PCR Utilized in a Commercial Dual-Target Diagnostic Assay
por: Artesi, Maria, et al.
Publicado: (2020)

Clinical evaluation of a new single-tube multiplex reverse transcription PCR assay for simultaneous detection of 11 respiratory viruses, Mycoplasma pneumoniae and Chlamydia in hospitalized children with acute respiratory infections
por: Zhao, Meng-chuan, et al.
Publicado: (2017)

Improved Molecular Diagnosis of COVID-19 by the Novel, Highly Sensitive and Specific COVID-19-RdRp/Hel Real-Time Reverse Transcription-PCR Assay Validated In Vitro and with Clinical Specimens
por: Chan, Jasper Fuk-Woo, et al.
Publicado: (2020)

Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I
por: OKINO, Cintia Hiromi, et al.
Publicado: (2018)

Development and application of multiplex PCR assays for detection of virus-induced respiratory disease complex in dogs
por: PIEWBANG, Chutchai, et al.
Publicado: (2016)

Impact of SARS-CoV-2 Variants on the Analytical Sensitivity of rRT-PCR Assays
por: Chen, Yuqing, et al.
Publicado: (2022)

Development of a PCR assay for detection and differentiation of Muscovy duck and goose parvoviruses based on NS gene characterization
por: WAN, Chunhe, et al.
Publicado: (2018)

Evaluation of PCR cycle threshold values by patient population with the quidel lyra SARS-CoV-2 assay
por: Potter, Robert F., et al.
Publicado: (2021)

Comparison of Two High-Throughput Reverse Transcription-PCR Systems for the Detection of Severe Acute Respiratory Syndrome Coronavirus 2
por: Craney, Arryn R., et al.
Publicado: (2020)

Inference of Active Viral Replication in Cases with Sustained Positive Reverse Transcription-PCR Results for SARS-CoV-2
por: Rodríguez-Grande, Cristina, et al.
Publicado: (2021)

Comparison of ePlex Respiratory Pathogen Panel with Laboratory-Developed Real-Time PCR Assays for Detection of Respiratory Pathogens
por: Nijhuis, R. H. T., et al.
Publicado: (2017)

Development of a Rapid Live SARS-CoV-2 Neutralization Assay Based on a qPCR Readout
por: Lichtenegger, Sabine, et al.
Publicado: (2022)

Evaluation of a Rapid and Accessible Reverse Transcription-Quantitative PCR Approach for SARS-CoV-2 Variant of Concern Identification
por: Yeung, Priscilla S.-W., et al.
Publicado: (2022)

Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus
por: Dauner, Allison L., et al.
Publicado: (2015)

Evaluation of Transport Media and Specimen Transport Conditions for the Detection of SARS-CoV-2 by Use of Real-Time Reverse Transcription-PCR
por: Rogers, Amy A., et al.
Publicado: (2020)

Pooling of Nasopharyngeal Swab Samples To Overcome a Global Shortage of Real-Time Reverse Transcription-PCR COVID-19 Test Kits
por: More, Sunil, et al.
Publicado: (2021)

Factors associated with weak positive SARS-CoV-2 diagnosis by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR)
por: Rawat, Priyank, et al.
Publicado: (2022)

Utility of a Stressed Single Nucleotide Polymorphism (SNP) Real-Time PCR Assay for Rapid Identification of Measles Vaccine Strains in Patient Samples
por: Tran, Thomas, et al.
Publicado: (2018)

Application of a SYBR® Green One Step Real-time RT-PCR Assay to Detect Type 1 Porcine Reproductive and Respiratory Syndrome Virus
por: ISEKI, Hiroshi, et al.
Publicado: (2014)

Development of a quantitative real-time RT-PCR assay for detecting Taiwan ferret badger rabies virus in ear tissue of ferret badgers and mice
por: HSU, Ai-Ping, et al.
Publicado: (2018)

Whole-genome sequences of influenza A(H3N2) viruses isolated from Brazilian patients with mild illness during the 2014 season
por: Resende, Paola Cristina, et al.
Publicado: (2015)

Single-Amplicon Multiplex Real-Time Reverse Transcription-PCR with Tiled Probes To Detect SARS-CoV-2 spike Mutations Associated with Variants of Concern
por: Babiker, Ahmed, et al.
Publicado: (2021)

Evaluation of the QIAstat-Dx Respiratory SARS-CoV-2 Panel, the First Rapid Multiplex PCR Commercial Assay for SARS-CoV-2 Detection
por: Visseaux, Benoit, et al.
Publicado: (2020)

Comparison of FilmArray Respiratory Panel and laboratory-developed real-time reverse transcription–polymerase chain reaction assays for respiratory virus detection()()
por: Renaud, Christian, et al.
Publicado: (2012)

Development and evaluation of multiplex real-time RT-PCR assays for seasonal, pandemic A/H1pdm09 and avian A/H5 influenza viruses detection
por: Choi, Jang-Hoon, et al.
Publicado: (2013)

Rapid High-Throughput Whole-Genome Sequencing of SARS-CoV-2 by Using One-Step Reverse Transcription-PCR Amplification with an Integrated Microfluidic System and Next-Generation Sequencing
por: Li, Tao, et al.
Publicado: (2021)

Assessment of a Novel Automatic Real-Time PCR Assay on the Cobas 4800 Analyzer as a Screening Platform for Hepatitis C Virus Genotyping in Clinical Practice: Comparison with Massive Sequencing
por: Nieto-Aponte, Leonardo, et al.
Publicado: (2017)

One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus
por: LIU, Dafei, et al.
Publicado: (2018)

Occurrence of bovine ephemeral fever in Okinawa Prefecture, Japan, in 2012 and development of a reverse-transcription polymerase chain reaction assay to detect bovine ephemeral fever virus gene
por: NIWA, Tsuyoshi, et al.
Publicado: (2014)

Simple and rapid detection of severe fever with thrombocytopenia syndrome virus in cats by reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay using a dried reagent
por: ISHIJIMA, Keita, et al.
Publicado: (2023)

Performance Characteristics of the Abbott BinaxNOW SARS-CoV-2 Antigen Test in Comparison to Real-Time Reverse Transcriptase PCR and Viral Culture in Community Testing Sites during November 2020
por: Almendares, Olivia, et al.
Publicado: (2022)

Plaque Assay for Murine Norovirus
por: Gonzalez-Hernandez, Mariam B., et al.
Publicado: (2012)

Isolation of epizootic hemorrhagic disease virus serotype 7 from cattle showing fever in Japan in 2016 and improvement of a reverse transcription-polymerase chain reaction assay to detect epizootic hemorrhagic disease virus
por: YAMAMOTO, Kunitaka, et al.
Publicado: (2021)

Quantitative PCR detection of feline morbillivirus in cat urine samples
por: FURUYA, Tetsuya, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_ub8nr8nqebk0bk087ufg4mt8mq