Palindromic sequence-targeted (PST) PCR: a rapid and efficient method for high-throughput gene characterization and genome walking

Genome walking (GW) refers to the capture and sequencing of unknown regions in a long DNA molecule that are adjacent to a region with a known sequence. A novel PCR-based method, palindromic sequence-targeted PCR (PST-PCR), was developed. PST-PCR is based on a distinctive design of walking primers an...

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Detalles Bibliográficos
Autores principales: Kalendar, Ruslan, Shustov, Alexandr V., Seppänen, Mervi M., Schulman, Alan H., Stoddard, Frederick L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6881309/
https://www.ncbi.nlm.nih.gov/pubmed/31776407
http://dx.doi.org/10.1038/s41598-019-54168-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6881309/
https://www.ncbi.nlm.nih.gov/pubmed/31776407
http://dx.doi.org/10.1038/s41598-019-54168-0

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