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High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato

CRISPR/Cas9 editing efficacies in tetraploid potato were highly improved through the use of endogenous potato U6 promoters. Highly increased editing efficiencies in the Granular Bound Starch Synthase gene at the protoplast level were obtained by replacement of the Arabidopsis U6 promotor, driving ex...

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Detalles Bibliográficos
Autores principales: Johansen, Ida Elisabeth, Liu, Ying, Jørgensen, Bodil, Bennett, Eric Paul, Andreasson, Erik, Nielsen, Kåre L., Blennow, Andreas, Petersen, Bent Larsen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6881354/
https://www.ncbi.nlm.nih.gov/pubmed/31776399
http://dx.doi.org/10.1038/s41598-019-54126-w