CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci

CRISPR-Cas9 is an efficient and versatile tool for genome engineering in many species. However, inducible CRISPR-Cas9 editing systems that regulate Cas9 activity or sgRNA expression often suffer from significant limitations, including reduced editing capacity, off-target effects, or leaky expression...

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Detalles Bibliográficos
Autores principales: Chylinski, Krzysztof, Hubmann, Maria, Hanna, Ruth E., Yanchus, Connor, Michlits, Georg, Uijttewaal, Esther C. H., Doench, John, Schramek, Daniel, Elling, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6884486/
https://www.ncbi.nlm.nih.gov/pubmed/31784531
http://dx.doi.org/10.1038/s41467-019-13403-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6884486/
https://www.ncbi.nlm.nih.gov/pubmed/31784531
http://dx.doi.org/10.1038/s41467-019-13403-y

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