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Ammonium Gemini Surfactants Form Complexes with Model Oligomers of siRNA and dsDNA

Dimeric cationic surfactants (gemini-type) are a group of amphiphilic compounds with potential use in gene therapy as effective carriers for nucleic acid transfection (i.e., siRNA, DNA, and plasmid DNA). Our studies have shown the formation of lipoplexes composed of alkanediyl-α,ω-bis[(oxymethyl)dim...

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Autores principales: Andrzejewska, Weronika, Wilkowska, Michalina, Skrzypczak, Andrzej, Kozak, Maciej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6887939/
https://www.ncbi.nlm.nih.gov/pubmed/31703275
http://dx.doi.org/10.3390/ijms20225546
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author Andrzejewska, Weronika
Wilkowska, Michalina
Skrzypczak, Andrzej
Kozak, Maciej
author_facet Andrzejewska, Weronika
Wilkowska, Michalina
Skrzypczak, Andrzej
Kozak, Maciej
author_sort Andrzejewska, Weronika
collection PubMed
description Dimeric cationic surfactants (gemini-type) are a group of amphiphilic compounds with potential use in gene therapy as effective carriers for nucleic acid transfection (i.e., siRNA, DNA, and plasmid DNA). Our studies have shown the formation of lipoplexes composed of alkanediyl-α,ω-bis[(oxymethyl)dimethyldodecylammonium] chlorides and selected 21-base-pair nucleic acid (dsDNA and siRNA) oligomers. To examine the structure and physicochemical properties of these systems, optical microscopy, circular dichroism spectroscopy (CD), small-angle X-ray scattering of synchrotron radiation (SR-SAXS), and agarose gel electrophoresis (AGE) were used. The lengths of spacer groups of the studied surfactants had a significant influence on the surfactants’ complexing properties. The lowest charge ratio (p/n) at which stable lipoplexes were observed was 1.5 and the most frequently occurring microstructure of these lipoplexes were cubic and micellar phases for dsDNA and siRNA, respectively. The cytotoxicity tests on HeLa cells indicated the non-toxic concentration of surfactants to be at approximately 10 µM. The dicationic gemini surfactants studied form complexes with siRNA and dsDNA oligomers; however, the complexation process is more effective towards siRNA. Therefore these systems could be applied as transfection systems for therapeutic nucleic acids.
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spelling pubmed-68879392019-12-09 Ammonium Gemini Surfactants Form Complexes with Model Oligomers of siRNA and dsDNA Andrzejewska, Weronika Wilkowska, Michalina Skrzypczak, Andrzej Kozak, Maciej Int J Mol Sci Article Dimeric cationic surfactants (gemini-type) are a group of amphiphilic compounds with potential use in gene therapy as effective carriers for nucleic acid transfection (i.e., siRNA, DNA, and plasmid DNA). Our studies have shown the formation of lipoplexes composed of alkanediyl-α,ω-bis[(oxymethyl)dimethyldodecylammonium] chlorides and selected 21-base-pair nucleic acid (dsDNA and siRNA) oligomers. To examine the structure and physicochemical properties of these systems, optical microscopy, circular dichroism spectroscopy (CD), small-angle X-ray scattering of synchrotron radiation (SR-SAXS), and agarose gel electrophoresis (AGE) were used. The lengths of spacer groups of the studied surfactants had a significant influence on the surfactants’ complexing properties. The lowest charge ratio (p/n) at which stable lipoplexes were observed was 1.5 and the most frequently occurring microstructure of these lipoplexes were cubic and micellar phases for dsDNA and siRNA, respectively. The cytotoxicity tests on HeLa cells indicated the non-toxic concentration of surfactants to be at approximately 10 µM. The dicationic gemini surfactants studied form complexes with siRNA and dsDNA oligomers; however, the complexation process is more effective towards siRNA. Therefore these systems could be applied as transfection systems for therapeutic nucleic acids. MDPI 2019-11-07 /pmc/articles/PMC6887939/ /pubmed/31703275 http://dx.doi.org/10.3390/ijms20225546 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Andrzejewska, Weronika
Wilkowska, Michalina
Skrzypczak, Andrzej
Kozak, Maciej
Ammonium Gemini Surfactants Form Complexes with Model Oligomers of siRNA and dsDNA
title Ammonium Gemini Surfactants Form Complexes with Model Oligomers of siRNA and dsDNA
title_full Ammonium Gemini Surfactants Form Complexes with Model Oligomers of siRNA and dsDNA
title_fullStr Ammonium Gemini Surfactants Form Complexes with Model Oligomers of siRNA and dsDNA
title_full_unstemmed Ammonium Gemini Surfactants Form Complexes with Model Oligomers of siRNA and dsDNA
title_short Ammonium Gemini Surfactants Form Complexes with Model Oligomers of siRNA and dsDNA
title_sort ammonium gemini surfactants form complexes with model oligomers of sirna and dsdna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6887939/
https://www.ncbi.nlm.nih.gov/pubmed/31703275
http://dx.doi.org/10.3390/ijms20225546
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