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Cloning and Expression Analysis of the BocMBF1c Gene Involved in Heat Tolerance in Chinese Kale

Chinese kale (Brassica oleracea var. chinensis Lei) is an important vegetable crop in South China, valued for its nutritional content and taste. Nonetheless, the thermal tolerance of Chinese kale still needs improvement. Molecular characterization of Chinese kale’s heat stress response could provide...

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Autores principales: Zou, Lifang, Yu, Bingwei, Ma, Xing-Liang, Cao, Bihao, Chen, Guoju, Chen, Changming, Lei, Jianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888671/
https://www.ncbi.nlm.nih.gov/pubmed/31718028
http://dx.doi.org/10.3390/ijms20225637
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author Zou, Lifang
Yu, Bingwei
Ma, Xing-Liang
Cao, Bihao
Chen, Guoju
Chen, Changming
Lei, Jianjun
author_facet Zou, Lifang
Yu, Bingwei
Ma, Xing-Liang
Cao, Bihao
Chen, Guoju
Chen, Changming
Lei, Jianjun
author_sort Zou, Lifang
collection PubMed
description Chinese kale (Brassica oleracea var. chinensis Lei) is an important vegetable crop in South China, valued for its nutritional content and taste. Nonetheless, the thermal tolerance of Chinese kale still needs improvement. Molecular characterization of Chinese kale’s heat stress response could provide a timely solution for developing a thermally tolerant Chinese kale variety. Here, we report the cloning of multi-protein bridging factor (MBF) 1c from Chinese kale (BocMBF1c), an ortholog to the key heat stress responsive gene MBF1c. Phylogenetic analysis showed that BocMBF1c is highly similar to the stress-response transcriptional coactivator MBF1c from Arabidopsis thaliana (AtMBF1c), and the BocMBF1c coding region conserves MBF1 and helix-turn-helix (HTH) domains. Moreover, the promoter region of BocMBF1c contains three heat shock elements (HSEs) and, thus, is highly responsive to heat treatment. This was verified in Nicotiana benthamiana leaf tissue using a green fluorescent protein (GFP) reporter. In addition, the expression of BocMBF1c can be induced by various abiotic stresses in Chinese kale which indicates the involvement of stress responses. The BocMBF1c-eGFP (enhanced green fluorescent protein) chimeric protein quickly translocated into the nucleus under high temperature treatment in Nicotiana benthamiana leaf tissue. Overexpression of BocMBF1c in Arabidopsis thaliana results in a larger size and enhanced thermal tolerance compared with the wild type. Our results provide valuable insight for the role of BocMBF1c during heat stress in Chinese kale.
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spelling pubmed-68886712019-12-09 Cloning and Expression Analysis of the BocMBF1c Gene Involved in Heat Tolerance in Chinese Kale Zou, Lifang Yu, Bingwei Ma, Xing-Liang Cao, Bihao Chen, Guoju Chen, Changming Lei, Jianjun Int J Mol Sci Article Chinese kale (Brassica oleracea var. chinensis Lei) is an important vegetable crop in South China, valued for its nutritional content and taste. Nonetheless, the thermal tolerance of Chinese kale still needs improvement. Molecular characterization of Chinese kale’s heat stress response could provide a timely solution for developing a thermally tolerant Chinese kale variety. Here, we report the cloning of multi-protein bridging factor (MBF) 1c from Chinese kale (BocMBF1c), an ortholog to the key heat stress responsive gene MBF1c. Phylogenetic analysis showed that BocMBF1c is highly similar to the stress-response transcriptional coactivator MBF1c from Arabidopsis thaliana (AtMBF1c), and the BocMBF1c coding region conserves MBF1 and helix-turn-helix (HTH) domains. Moreover, the promoter region of BocMBF1c contains three heat shock elements (HSEs) and, thus, is highly responsive to heat treatment. This was verified in Nicotiana benthamiana leaf tissue using a green fluorescent protein (GFP) reporter. In addition, the expression of BocMBF1c can be induced by various abiotic stresses in Chinese kale which indicates the involvement of stress responses. The BocMBF1c-eGFP (enhanced green fluorescent protein) chimeric protein quickly translocated into the nucleus under high temperature treatment in Nicotiana benthamiana leaf tissue. Overexpression of BocMBF1c in Arabidopsis thaliana results in a larger size and enhanced thermal tolerance compared with the wild type. Our results provide valuable insight for the role of BocMBF1c during heat stress in Chinese kale. MDPI 2019-11-11 /pmc/articles/PMC6888671/ /pubmed/31718028 http://dx.doi.org/10.3390/ijms20225637 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zou, Lifang
Yu, Bingwei
Ma, Xing-Liang
Cao, Bihao
Chen, Guoju
Chen, Changming
Lei, Jianjun
Cloning and Expression Analysis of the BocMBF1c Gene Involved in Heat Tolerance in Chinese Kale
title Cloning and Expression Analysis of the BocMBF1c Gene Involved in Heat Tolerance in Chinese Kale
title_full Cloning and Expression Analysis of the BocMBF1c Gene Involved in Heat Tolerance in Chinese Kale
title_fullStr Cloning and Expression Analysis of the BocMBF1c Gene Involved in Heat Tolerance in Chinese Kale
title_full_unstemmed Cloning and Expression Analysis of the BocMBF1c Gene Involved in Heat Tolerance in Chinese Kale
title_short Cloning and Expression Analysis of the BocMBF1c Gene Involved in Heat Tolerance in Chinese Kale
title_sort cloning and expression analysis of the bocmbf1c gene involved in heat tolerance in chinese kale
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888671/
https://www.ncbi.nlm.nih.gov/pubmed/31718028
http://dx.doi.org/10.3390/ijms20225637
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