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Transcriptome changes in undifferentiated Caco-2 cells exposed to food-grade titanium dioxide (E171): contribution of the nano- and micro- sized particles

The food additive titanium dioxide (TiO(2)), or E171, is a white food colorant. Recent studies showed after E171 ingestion a significantly increased number of colorectal tumours in a colorectal cancer mouse model as well as inflammatory responses and dysregulation of the immune system in the intesti...

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Autores principales: Proquin, Héloïse, Jonkhout, Marloes C. M., Jetten, Marlon J., van Loveren, Henk, de Kok, Theo M., Briedé, Jacob J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893026/
https://www.ncbi.nlm.nih.gov/pubmed/31797963
http://dx.doi.org/10.1038/s41598-019-54675-0
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author Proquin, Héloïse
Jonkhout, Marloes C. M.
Jetten, Marlon J.
van Loveren, Henk
de Kok, Theo M.
Briedé, Jacob J.
author_facet Proquin, Héloïse
Jonkhout, Marloes C. M.
Jetten, Marlon J.
van Loveren, Henk
de Kok, Theo M.
Briedé, Jacob J.
author_sort Proquin, Héloïse
collection PubMed
description The food additive titanium dioxide (TiO(2)), or E171, is a white food colorant. Recent studies showed after E171 ingestion a significantly increased number of colorectal tumours in a colorectal cancer mouse model as well as inflammatory responses and dysregulation of the immune system in the intestine of rats. In the mouse colon, E171 induced gene expression changes related to oxidative stress, impairment of the immune system, activation of signalling and cancer-related processes. E171 comprises nanoparticles (NPs) and microparticles (MPs). Previous in vitro studies showed that E171, NPs and MPs induced oxidative stress responses, DNA damage and micronuclei formation. This study aimed to investigate the relative contribution of the NPs and MPs to effects of E171 at the transcriptome level in undifferentiated Caco-2 cells by genome wide microarray analysis. The results showed that E171, NPs, and MPs induce gene expression changes related to signalling, inflammation, immune system, transport and cancer. At the pathway level, metabolism of proteins with the insulin processing pathway and haemostasis were specific to E171 exposure. The gene expression changes associated with the immune system and inflammation induced by E171, MPs, and NPs suggest the creation of a favourable environment for colon cancer development.
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spelling pubmed-68930262019-12-11 Transcriptome changes in undifferentiated Caco-2 cells exposed to food-grade titanium dioxide (E171): contribution of the nano- and micro- sized particles Proquin, Héloïse Jonkhout, Marloes C. M. Jetten, Marlon J. van Loveren, Henk de Kok, Theo M. Briedé, Jacob J. Sci Rep Article The food additive titanium dioxide (TiO(2)), or E171, is a white food colorant. Recent studies showed after E171 ingestion a significantly increased number of colorectal tumours in a colorectal cancer mouse model as well as inflammatory responses and dysregulation of the immune system in the intestine of rats. In the mouse colon, E171 induced gene expression changes related to oxidative stress, impairment of the immune system, activation of signalling and cancer-related processes. E171 comprises nanoparticles (NPs) and microparticles (MPs). Previous in vitro studies showed that E171, NPs and MPs induced oxidative stress responses, DNA damage and micronuclei formation. This study aimed to investigate the relative contribution of the NPs and MPs to effects of E171 at the transcriptome level in undifferentiated Caco-2 cells by genome wide microarray analysis. The results showed that E171, NPs, and MPs induce gene expression changes related to signalling, inflammation, immune system, transport and cancer. At the pathway level, metabolism of proteins with the insulin processing pathway and haemostasis were specific to E171 exposure. The gene expression changes associated with the immune system and inflammation induced by E171, MPs, and NPs suggest the creation of a favourable environment for colon cancer development. Nature Publishing Group UK 2019-12-04 /pmc/articles/PMC6893026/ /pubmed/31797963 http://dx.doi.org/10.1038/s41598-019-54675-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Proquin, Héloïse
Jonkhout, Marloes C. M.
Jetten, Marlon J.
van Loveren, Henk
de Kok, Theo M.
Briedé, Jacob J.
Transcriptome changes in undifferentiated Caco-2 cells exposed to food-grade titanium dioxide (E171): contribution of the nano- and micro- sized particles
title Transcriptome changes in undifferentiated Caco-2 cells exposed to food-grade titanium dioxide (E171): contribution of the nano- and micro- sized particles
title_full Transcriptome changes in undifferentiated Caco-2 cells exposed to food-grade titanium dioxide (E171): contribution of the nano- and micro- sized particles
title_fullStr Transcriptome changes in undifferentiated Caco-2 cells exposed to food-grade titanium dioxide (E171): contribution of the nano- and micro- sized particles
title_full_unstemmed Transcriptome changes in undifferentiated Caco-2 cells exposed to food-grade titanium dioxide (E171): contribution of the nano- and micro- sized particles
title_short Transcriptome changes in undifferentiated Caco-2 cells exposed to food-grade titanium dioxide (E171): contribution of the nano- and micro- sized particles
title_sort transcriptome changes in undifferentiated caco-2 cells exposed to food-grade titanium dioxide (e171): contribution of the nano- and micro- sized particles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893026/
https://www.ncbi.nlm.nih.gov/pubmed/31797963
http://dx.doi.org/10.1038/s41598-019-54675-0
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