Cargando…
Combined Buckwheat d-Fagomine and Fish Omega-3 PUFAs Stabilize the Populations of Gut Prevotella and Bacteroides While Reducing Weight Gain in Rats
Some functional food components may help maintain homeostasis by promoting balanced gut microbiota. Here, we explore the possible complementary effects of d-fagomine and ω-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA 1:1) on putatively beneficial gut...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893428/ https://www.ncbi.nlm.nih.gov/pubmed/31683529 http://dx.doi.org/10.3390/nu11112606 |
_version_ | 1783476196624826368 |
---|---|
author | Hereu, Mercè Ramos-Romero, Sara Marín-Valls, Roser Amézqueta, Susana Miralles-Pérez, Bernat Romeu, Marta Méndez, Lucía Medina, Isabel Torres, Josep Lluís |
author_facet | Hereu, Mercè Ramos-Romero, Sara Marín-Valls, Roser Amézqueta, Susana Miralles-Pérez, Bernat Romeu, Marta Méndez, Lucía Medina, Isabel Torres, Josep Lluís |
author_sort | Hereu, Mercè |
collection | PubMed |
description | Some functional food components may help maintain homeostasis by promoting balanced gut microbiota. Here, we explore the possible complementary effects of d-fagomine and ω-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA 1:1) on putatively beneficial gut bacterial strains. Male Sprague–Dawley rats were supplemented with d-fagomine, ω-3 PUFAs, or both, for 23 weeks. Bacterial subgroups were evaluated in fecal DNA by quantitative real-time polymerase chain reaction (qRT-PCR) and short-chain fatty acids were determined by gas chromatography. We found that the populations of the genus Prevotella remained stable over time in animals supplemented with d-fagomine, independently of ω-3 PUFA supplementation. Animals in these groups gained less weight than controls and rats given only ω-3 PUFAs. d-Fagomine supplementation together with ω-3 PUFAs maintained the relative populations of Bacteroides. ω-3 PUFAs alone or combined with d-fagomine reduced the amount of acetic acid and total short-chain fatty acids in feces. The plasma levels of pro-inflammatory arachidonic acid derived metabolites, triglycerides and cholesterol were lower in both groups supplemented with ω-3 PUFAs. The d-fagomine and ω-3 PUFAs combination provided the functional benefits of each supplement. Notably, it helped stabilize populations of Prevotella in the rat intestinal tract while reducing weight gain and providing the anti-inflammatory and cardiovascular benefits of ω-3 PUFAs. |
format | Online Article Text |
id | pubmed-6893428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68934282019-12-23 Combined Buckwheat d-Fagomine and Fish Omega-3 PUFAs Stabilize the Populations of Gut Prevotella and Bacteroides While Reducing Weight Gain in Rats Hereu, Mercè Ramos-Romero, Sara Marín-Valls, Roser Amézqueta, Susana Miralles-Pérez, Bernat Romeu, Marta Méndez, Lucía Medina, Isabel Torres, Josep Lluís Nutrients Article Some functional food components may help maintain homeostasis by promoting balanced gut microbiota. Here, we explore the possible complementary effects of d-fagomine and ω-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA 1:1) on putatively beneficial gut bacterial strains. Male Sprague–Dawley rats were supplemented with d-fagomine, ω-3 PUFAs, or both, for 23 weeks. Bacterial subgroups were evaluated in fecal DNA by quantitative real-time polymerase chain reaction (qRT-PCR) and short-chain fatty acids were determined by gas chromatography. We found that the populations of the genus Prevotella remained stable over time in animals supplemented with d-fagomine, independently of ω-3 PUFA supplementation. Animals in these groups gained less weight than controls and rats given only ω-3 PUFAs. d-Fagomine supplementation together with ω-3 PUFAs maintained the relative populations of Bacteroides. ω-3 PUFAs alone or combined with d-fagomine reduced the amount of acetic acid and total short-chain fatty acids in feces. The plasma levels of pro-inflammatory arachidonic acid derived metabolites, triglycerides and cholesterol were lower in both groups supplemented with ω-3 PUFAs. The d-fagomine and ω-3 PUFAs combination provided the functional benefits of each supplement. Notably, it helped stabilize populations of Prevotella in the rat intestinal tract while reducing weight gain and providing the anti-inflammatory and cardiovascular benefits of ω-3 PUFAs. MDPI 2019-10-31 /pmc/articles/PMC6893428/ /pubmed/31683529 http://dx.doi.org/10.3390/nu11112606 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hereu, Mercè Ramos-Romero, Sara Marín-Valls, Roser Amézqueta, Susana Miralles-Pérez, Bernat Romeu, Marta Méndez, Lucía Medina, Isabel Torres, Josep Lluís Combined Buckwheat d-Fagomine and Fish Omega-3 PUFAs Stabilize the Populations of Gut Prevotella and Bacteroides While Reducing Weight Gain in Rats |
title | Combined Buckwheat d-Fagomine and Fish Omega-3 PUFAs Stabilize the Populations of Gut Prevotella and Bacteroides While Reducing Weight Gain in Rats |
title_full | Combined Buckwheat d-Fagomine and Fish Omega-3 PUFAs Stabilize the Populations of Gut Prevotella and Bacteroides While Reducing Weight Gain in Rats |
title_fullStr | Combined Buckwheat d-Fagomine and Fish Omega-3 PUFAs Stabilize the Populations of Gut Prevotella and Bacteroides While Reducing Weight Gain in Rats |
title_full_unstemmed | Combined Buckwheat d-Fagomine and Fish Omega-3 PUFAs Stabilize the Populations of Gut Prevotella and Bacteroides While Reducing Weight Gain in Rats |
title_short | Combined Buckwheat d-Fagomine and Fish Omega-3 PUFAs Stabilize the Populations of Gut Prevotella and Bacteroides While Reducing Weight Gain in Rats |
title_sort | combined buckwheat d-fagomine and fish omega-3 pufas stabilize the populations of gut prevotella and bacteroides while reducing weight gain in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893428/ https://www.ncbi.nlm.nih.gov/pubmed/31683529 http://dx.doi.org/10.3390/nu11112606 |
work_keys_str_mv | AT hereumerce combinedbuckwheatdfagomineandfishomega3pufasstabilizethepopulationsofgutprevotellaandbacteroideswhilereducingweightgaininrats AT ramosromerosara combinedbuckwheatdfagomineandfishomega3pufasstabilizethepopulationsofgutprevotellaandbacteroideswhilereducingweightgaininrats AT marinvallsroser combinedbuckwheatdfagomineandfishomega3pufasstabilizethepopulationsofgutprevotellaandbacteroideswhilereducingweightgaininrats AT amezquetasusana combinedbuckwheatdfagomineandfishomega3pufasstabilizethepopulationsofgutprevotellaandbacteroideswhilereducingweightgaininrats AT mirallesperezbernat combinedbuckwheatdfagomineandfishomega3pufasstabilizethepopulationsofgutprevotellaandbacteroideswhilereducingweightgaininrats AT romeumarta combinedbuckwheatdfagomineandfishomega3pufasstabilizethepopulationsofgutprevotellaandbacteroideswhilereducingweightgaininrats AT mendezlucia combinedbuckwheatdfagomineandfishomega3pufasstabilizethepopulationsofgutprevotellaandbacteroideswhilereducingweightgaininrats AT medinaisabel combinedbuckwheatdfagomineandfishomega3pufasstabilizethepopulationsofgutprevotellaandbacteroideswhilereducingweightgaininrats AT torresjoseplluis combinedbuckwheatdfagomineandfishomega3pufasstabilizethepopulationsofgutprevotellaandbacteroideswhilereducingweightgaininrats |