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Housekeeping gene validation for RT-qPCR studies on synovial fibroblasts derived from healthy and osteoarthritic patients with focus on mechanical loading

Selection of appropriate housekeeping genes is essential for the validity of data normalization in reverse transcription quantitative PCR (RT-qPCR). Synovial fibroblasts (SF) play a mediating role in the development and progression of osteoarthritis (OA) pathogenesis, but there is no information on...

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Detalles Bibliográficos
Autores principales: Nazet, Ute, Schröder, Agnes, Grässel, Susanne, Muschter, Dominique, Proff, Peter, Kirschneck, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6897414/
https://www.ncbi.nlm.nih.gov/pubmed/31809510
http://dx.doi.org/10.1371/journal.pone.0225790