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Housekeeping gene validation for RT-qPCR studies on synovial fibroblasts derived from healthy and osteoarthritic patients with focus on mechanical loading
Selection of appropriate housekeeping genes is essential for the validity of data normalization in reverse transcription quantitative PCR (RT-qPCR). Synovial fibroblasts (SF) play a mediating role in the development and progression of osteoarthritis (OA) pathogenesis, but there is no information on...
Autores principales: | Nazet, Ute, Schröder, Agnes, Grässel, Susanne, Muschter, Dominique, Proff, Peter, Kirschneck, Christian |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6897414/ https://www.ncbi.nlm.nih.gov/pubmed/31809510 http://dx.doi.org/10.1371/journal.pone.0225790 |
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