Fast and cloning‐free CRISPR/Cas9‐mediated genomic editing in mammalian cells

CHoP‐In (CRISPR/Cas9‐mediated Homology‐independent PCR‐product integration) is a fast, non‐homologous end‐joining based, strategy for genomic editing in mammalian cells. There is no requirement for cloning in generation of the integration donor, instead the desired integration donor is produced as a...

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Detalles Bibliográficos
Autores principales: Manna, Paul T., Davis, Luther J., Robinson, Margaret S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons A/S 2019
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6899835/
https://www.ncbi.nlm.nih.gov/pubmed/31503392
http://dx.doi.org/10.1111/tra.12696

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6899835/
https://www.ncbi.nlm.nih.gov/pubmed/31503392
http://dx.doi.org/10.1111/tra.12696

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