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Fast and cloning‐free CRISPR/Cas9‐mediated genomic editing in mammalian cells
CHoP‐In (CRISPR/Cas9‐mediated Homology‐independent PCR‐product integration) is a fast, non‐homologous end‐joining based, strategy for genomic editing in mammalian cells. There is no requirement for cloning in generation of the integration donor, instead the desired integration donor is produced as a...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley & Sons A/S
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6899835/ https://www.ncbi.nlm.nih.gov/pubmed/31503392 http://dx.doi.org/10.1111/tra.12696 |