A Fluorescent Activatable AND‐Gate Chemokine CCL2 Enables In Vivo Detection of Metastasis‐Associated Macrophages

We report the novel chemical design of fluorescent activatable chemokines as highly specific functional probes for imaging subpopulations of immune cells in live tumours. Activatable chemokines behave as AND‐gates since they emit only after receptor binding and intracellular activation, showing enha...

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Detalles Bibliográficos
Autores principales: Fernandez, Antonio, Thompson, Emily J., Pollard, Jeffrey W., Kitamura, Takanori, Vendrell, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6900180/
https://www.ncbi.nlm.nih.gov/pubmed/31535788
http://dx.doi.org/10.1002/anie.201910955
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author Fernandez, Antonio
Thompson, Emily J.
Pollard, Jeffrey W.
Kitamura, Takanori
Vendrell, Marc
author_facet Fernandez, Antonio
Thompson, Emily J.
Pollard, Jeffrey W.
Kitamura, Takanori
Vendrell, Marc
author_sort Fernandez, Antonio
collection PubMed
description We report the novel chemical design of fluorescent activatable chemokines as highly specific functional probes for imaging subpopulations of immune cells in live tumours. Activatable chemokines behave as AND‐gates since they emit only after receptor binding and intracellular activation, showing enhanced selectivity over existing agents. We have applied this strategy to produce mCCL2‐MAF as the first probe for in vivo detection of metastasis‐associated macrophages in a preclinical model of lung metastasis. This strategy will accelerate the preparation of new chemokine‐based probes for imaging immune cell function in tumours.
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spelling pubmed-69001802019-12-20 A Fluorescent Activatable AND‐Gate Chemokine CCL2 Enables In Vivo Detection of Metastasis‐Associated Macrophages Fernandez, Antonio Thompson, Emily J. Pollard, Jeffrey W. Kitamura, Takanori Vendrell, Marc Angew Chem Int Ed Engl Communications We report the novel chemical design of fluorescent activatable chemokines as highly specific functional probes for imaging subpopulations of immune cells in live tumours. Activatable chemokines behave as AND‐gates since they emit only after receptor binding and intracellular activation, showing enhanced selectivity over existing agents. We have applied this strategy to produce mCCL2‐MAF as the first probe for in vivo detection of metastasis‐associated macrophages in a preclinical model of lung metastasis. This strategy will accelerate the preparation of new chemokine‐based probes for imaging immune cell function in tumours. John Wiley and Sons Inc. 2019-10-11 2019-11-18 /pmc/articles/PMC6900180/ /pubmed/31535788 http://dx.doi.org/10.1002/anie.201910955 Text en © 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Communications
Fernandez, Antonio
Thompson, Emily J.
Pollard, Jeffrey W.
Kitamura, Takanori
Vendrell, Marc
A Fluorescent Activatable AND‐Gate Chemokine CCL2 Enables In Vivo Detection of Metastasis‐Associated Macrophages
title A Fluorescent Activatable AND‐Gate Chemokine CCL2 Enables In Vivo Detection of Metastasis‐Associated Macrophages
title_full A Fluorescent Activatable AND‐Gate Chemokine CCL2 Enables In Vivo Detection of Metastasis‐Associated Macrophages
title_fullStr A Fluorescent Activatable AND‐Gate Chemokine CCL2 Enables In Vivo Detection of Metastasis‐Associated Macrophages
title_full_unstemmed A Fluorescent Activatable AND‐Gate Chemokine CCL2 Enables In Vivo Detection of Metastasis‐Associated Macrophages
title_short A Fluorescent Activatable AND‐Gate Chemokine CCL2 Enables In Vivo Detection of Metastasis‐Associated Macrophages
title_sort fluorescent activatable and‐gate chemokine ccl2 enables in vivo detection of metastasis‐associated macrophages
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6900180/
https://www.ncbi.nlm.nih.gov/pubmed/31535788
http://dx.doi.org/10.1002/anie.201910955
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