A nuclease-polymerase chain reaction enables amplification of probes used for capture-based DNA target enrichment

DNA target enrichment via hybridization capture is a commonly adopted approach which remains expensive due in-part to using biotinylated-probe panels. Here we provide a novel isothermal amplification reaction to amplify rapidly existing probe panels without knowledge of the sequences involved, there...

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Detalles Bibliográficos
Autores principales: Leong, Ka Wai, Yu, Fangyan, Adalsteinsson, Viktor A, Reed, Sarah, Gydush, Gregory, Ladas, Ioannis, Li, Jiang, Tantisira, Kelan G, Makrigiorgos, Gerassimos Mike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6902007/
https://www.ncbi.nlm.nih.gov/pubmed/31598677
http://dx.doi.org/10.1093/nar/gkz870

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6902007/
https://www.ncbi.nlm.nih.gov/pubmed/31598677
http://dx.doi.org/10.1093/nar/gkz870

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