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A nuclease-polymerase chain reaction enables amplification of probes used for capture-based DNA target enrichment
DNA target enrichment via hybridization capture is a commonly adopted approach which remains expensive due in-part to using biotinylated-probe panels. Here we provide a novel isothermal amplification reaction to amplify rapidly existing probe panels without knowledge of the sequences involved, there...
Autores principales: | Leong, Ka Wai, Yu, Fangyan, Adalsteinsson, Viktor A, Reed, Sarah, Gydush, Gregory, Ladas, Ioannis, Li, Jiang, Tantisira, Kelan G, Makrigiorgos, Gerassimos Mike |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6902007/ https://www.ncbi.nlm.nih.gov/pubmed/31598677 http://dx.doi.org/10.1093/nar/gkz870 |
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