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Carotenoid Production by Rhodotorula mucilaginosa in Batch and Fed-Batch Fermentation Using Agroindustrial Byproducts

Carotenoids are natural pigments that can be produced through biotechnological processes. However, the costs are relatively high and can be minimized by using lower-cost substrates as alternative nutrient sources. The fed-batch fermentation is one of the techniques used to obtain a high biomass conc...

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Autores principales: Dias Rodrigues, Tábita Veiga, Amore, Thalita D., Teixeira, Erika Carvalho, de Medeiros Burkert, Janaina Fernandes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: University of Zagreb Faculty of Food Technology and Biotechnology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6902290/
https://www.ncbi.nlm.nih.gov/pubmed/31866752
http://dx.doi.org/10.17113/ftb.57.03.19.6068
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author Dias Rodrigues, Tábita Veiga
Amore, Thalita D.
Teixeira, Erika Carvalho
de Medeiros Burkert, Janaina Fernandes
author_facet Dias Rodrigues, Tábita Veiga
Amore, Thalita D.
Teixeira, Erika Carvalho
de Medeiros Burkert, Janaina Fernandes
author_sort Dias Rodrigues, Tábita Veiga
collection PubMed
description Carotenoids are natural pigments that can be produced through biotechnological processes. However, the costs are relatively high and can be minimized by using lower-cost substrates as alternative nutrient sources. The fed-batch fermentation is one of the techniques used to obtain a high biomass concentration and/or maximum production. Thus, the aim of this work is to produce carotenoids in batch and fed-batch fermentation with the yeast Rhodotorula mucilaginosa CCT 7688 using agroindustrial byproducts in the culture medium. Carotenoid production was increased using experimental designs, which modified the concentration of the agroindustrial medium. In batch production the highest concentrations of total carotenoids (1248.5 μg/L) and biomass (7.9 g/L) were obtained in the medium containing 70 g/L sugar cane molasses and 3.4 g/L corn steep liquor at 25 °C and 180 rpm in 168 h, demonstrating an increase of 17% when compared to the standard yeast malt medium (1200 μg/L). In the fed-batch production, different feeding strategies were tested with 30 g/L sugar cane molasses and 6.5 g/L corn steep liquor, reaching a total carotenoid production of 3726 μg/L and biomass concentration of 16 g/L. Therefore, the strategy of the fed-batch process resulted in an increase in the carotenoid production of approx. 400% compared to that in the batch process (740.3 μg/L). Thus, the R. mucilaginosa strain has the potential to produce carotenoids in agroindustrial medium.
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spelling pubmed-69022902019-12-20 Carotenoid Production by Rhodotorula mucilaginosa in Batch and Fed-Batch Fermentation Using Agroindustrial Byproducts Dias Rodrigues, Tábita Veiga Amore, Thalita D. Teixeira, Erika Carvalho de Medeiros Burkert, Janaina Fernandes Food Technol Biotechnol Original Scientific Papers Carotenoids are natural pigments that can be produced through biotechnological processes. However, the costs are relatively high and can be minimized by using lower-cost substrates as alternative nutrient sources. The fed-batch fermentation is one of the techniques used to obtain a high biomass concentration and/or maximum production. Thus, the aim of this work is to produce carotenoids in batch and fed-batch fermentation with the yeast Rhodotorula mucilaginosa CCT 7688 using agroindustrial byproducts in the culture medium. Carotenoid production was increased using experimental designs, which modified the concentration of the agroindustrial medium. In batch production the highest concentrations of total carotenoids (1248.5 μg/L) and biomass (7.9 g/L) were obtained in the medium containing 70 g/L sugar cane molasses and 3.4 g/L corn steep liquor at 25 °C and 180 rpm in 168 h, demonstrating an increase of 17% when compared to the standard yeast malt medium (1200 μg/L). In the fed-batch production, different feeding strategies were tested with 30 g/L sugar cane molasses and 6.5 g/L corn steep liquor, reaching a total carotenoid production of 3726 μg/L and biomass concentration of 16 g/L. Therefore, the strategy of the fed-batch process resulted in an increase in the carotenoid production of approx. 400% compared to that in the batch process (740.3 μg/L). Thus, the R. mucilaginosa strain has the potential to produce carotenoids in agroindustrial medium. University of Zagreb Faculty of Food Technology and Biotechnology 2019-09 /pmc/articles/PMC6902290/ /pubmed/31866752 http://dx.doi.org/10.17113/ftb.57.03.19.6068 Text en http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 4.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Scientific Papers
Dias Rodrigues, Tábita Veiga
Amore, Thalita D.
Teixeira, Erika Carvalho
de Medeiros Burkert, Janaina Fernandes
Carotenoid Production by Rhodotorula mucilaginosa in Batch and Fed-Batch Fermentation Using Agroindustrial Byproducts
title Carotenoid Production by Rhodotorula mucilaginosa in Batch and Fed-Batch Fermentation Using Agroindustrial Byproducts
title_full Carotenoid Production by Rhodotorula mucilaginosa in Batch and Fed-Batch Fermentation Using Agroindustrial Byproducts
title_fullStr Carotenoid Production by Rhodotorula mucilaginosa in Batch and Fed-Batch Fermentation Using Agroindustrial Byproducts
title_full_unstemmed Carotenoid Production by Rhodotorula mucilaginosa in Batch and Fed-Batch Fermentation Using Agroindustrial Byproducts
title_short Carotenoid Production by Rhodotorula mucilaginosa in Batch and Fed-Batch Fermentation Using Agroindustrial Byproducts
title_sort carotenoid production by rhodotorula mucilaginosa in batch and fed-batch fermentation using agroindustrial byproducts
topic Original Scientific Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6902290/
https://www.ncbi.nlm.nih.gov/pubmed/31866752
http://dx.doi.org/10.17113/ftb.57.03.19.6068
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