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Purification of the recombinant green fluorescent protein from tobacco plants using alcohol/salt aqueous two-phase system and hydrophobic interaction chromatography

BACKGROUND: The green fluorescent protein (GFP) has been regarded as a valuable tool and widely applied as a biomarker in medical applications and diagnostics. A cost-efficient upstream expression system and an inexpensive downstream purification process will meet the demands of the GFP protein with...

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Detalles Bibliográficos
Autores principales: Dong, Jie, Ding, Xiangzhen, Wang, Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6902424/
https://www.ncbi.nlm.nih.gov/pubmed/31818280
http://dx.doi.org/10.1186/s12896-019-0590-y