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Purification of the recombinant green fluorescent protein from tobacco plants using alcohol/salt aqueous two-phase system and hydrophobic interaction chromatography
BACKGROUND: The green fluorescent protein (GFP) has been regarded as a valuable tool and widely applied as a biomarker in medical applications and diagnostics. A cost-efficient upstream expression system and an inexpensive downstream purification process will meet the demands of the GFP protein with...
Autores principales: | Dong, Jie, Ding, Xiangzhen, Wang, Sheng |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6902424/ https://www.ncbi.nlm.nih.gov/pubmed/31818280 http://dx.doi.org/10.1186/s12896-019-0590-y |
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