Cargando…
Induction of G0/G1 phase arrest and apoptosis by CRISPR/Cas9-mediated knockout of CDK2 in A375 melanocytes
Cutaneous melanoma is one of the most common malignant skin tumors, with a continuously increasing incidence. Cyclin-dependent kinase (CDK) 2 is a key regulator of G1-S transition and modulation of G2 progression; however, its role in cancer is a matter of debate. In the present study, a lentivirus...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904871/ https://www.ncbi.nlm.nih.gov/pubmed/31832188 http://dx.doi.org/10.3892/mco.2019.1952 |
_version_ | 1783478067930333184 |
---|---|
author | Liu, Houguang Li, Zheng Huo, Shanshan Wei, Qiongling Ge, Ling |
author_facet | Liu, Houguang Li, Zheng Huo, Shanshan Wei, Qiongling Ge, Ling |
author_sort | Liu, Houguang |
collection | PubMed |
description | Cutaneous melanoma is one of the most common malignant skin tumors, with a continuously increasing incidence. Cyclin-dependent kinase (CDK) 2 is a key regulator of G1-S transition and modulation of G2 progression; however, its role in cancer is a matter of debate. In the present study, a lentivirus expressing single-guide RNA (sgRNA) was constructed to knock out CDK2 using CRISP/Cas9 technology, in order to confirm the role of CDK2 in A375 human melanoma cells. The results demonstrated that CDK2 knockout induced G0/G1 phase arrest and early apoptosis by downregulating the expression of CDK4 and cyclin A2, and by upregulating the expression of cyclin D1. These results suggest that therapeutic strategies designed to target CDK2 using CRISP/Cas9 may improve the treatment outcome of cutaneous melanoma. |
format | Online Article Text |
id | pubmed-6904871 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-69048712019-12-12 Induction of G0/G1 phase arrest and apoptosis by CRISPR/Cas9-mediated knockout of CDK2 in A375 melanocytes Liu, Houguang Li, Zheng Huo, Shanshan Wei, Qiongling Ge, Ling Mol Clin Oncol Articles Cutaneous melanoma is one of the most common malignant skin tumors, with a continuously increasing incidence. Cyclin-dependent kinase (CDK) 2 is a key regulator of G1-S transition and modulation of G2 progression; however, its role in cancer is a matter of debate. In the present study, a lentivirus expressing single-guide RNA (sgRNA) was constructed to knock out CDK2 using CRISP/Cas9 technology, in order to confirm the role of CDK2 in A375 human melanoma cells. The results demonstrated that CDK2 knockout induced G0/G1 phase arrest and early apoptosis by downregulating the expression of CDK4 and cyclin A2, and by upregulating the expression of cyclin D1. These results suggest that therapeutic strategies designed to target CDK2 using CRISP/Cas9 may improve the treatment outcome of cutaneous melanoma. D.A. Spandidos 2020-01 2019-11-20 /pmc/articles/PMC6904871/ /pubmed/31832188 http://dx.doi.org/10.3892/mco.2019.1952 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Liu, Houguang Li, Zheng Huo, Shanshan Wei, Qiongling Ge, Ling Induction of G0/G1 phase arrest and apoptosis by CRISPR/Cas9-mediated knockout of CDK2 in A375 melanocytes |
title | Induction of G0/G1 phase arrest and apoptosis by CRISPR/Cas9-mediated knockout of CDK2 in A375 melanocytes |
title_full | Induction of G0/G1 phase arrest and apoptosis by CRISPR/Cas9-mediated knockout of CDK2 in A375 melanocytes |
title_fullStr | Induction of G0/G1 phase arrest and apoptosis by CRISPR/Cas9-mediated knockout of CDK2 in A375 melanocytes |
title_full_unstemmed | Induction of G0/G1 phase arrest and apoptosis by CRISPR/Cas9-mediated knockout of CDK2 in A375 melanocytes |
title_short | Induction of G0/G1 phase arrest and apoptosis by CRISPR/Cas9-mediated knockout of CDK2 in A375 melanocytes |
title_sort | induction of g0/g1 phase arrest and apoptosis by crispr/cas9-mediated knockout of cdk2 in a375 melanocytes |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904871/ https://www.ncbi.nlm.nih.gov/pubmed/31832188 http://dx.doi.org/10.3892/mco.2019.1952 |
work_keys_str_mv | AT liuhouguang inductionofg0g1phasearrestandapoptosisbycrisprcas9mediatedknockoutofcdk2ina375melanocytes AT lizheng inductionofg0g1phasearrestandapoptosisbycrisprcas9mediatedknockoutofcdk2ina375melanocytes AT huoshanshan inductionofg0g1phasearrestandapoptosisbycrisprcas9mediatedknockoutofcdk2ina375melanocytes AT weiqiongling inductionofg0g1phasearrestandapoptosisbycrisprcas9mediatedknockoutofcdk2ina375melanocytes AT geling inductionofg0g1phasearrestandapoptosisbycrisprcas9mediatedknockoutofcdk2ina375melanocytes |