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G3BP1 inhibits RNA virus replication by positively regulating RIG-I-mediated cellular antiviral response

Retinoic acid-inducible gene I (RIG-I) is a pattern recognition receptor and is involved in the innate immune response against RNA viruses infection. Here, we demonstrate that the Ras-GTPase-activating protein SH3-domain-binding protein 1 (G3BP1) serves as a positive regulator of the RIG-I-mediated...

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Autores principales: Yang, Wenping, Ru, Yi, Ren, Jingjing, Bai, Juncui, Wei, Junshu, Fu, Shaozu, Liu, Xiangtao, Li, Dan, Zheng, Haixue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906297/
https://www.ncbi.nlm.nih.gov/pubmed/31827077
http://dx.doi.org/10.1038/s41419-019-2178-9
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author Yang, Wenping
Ru, Yi
Ren, Jingjing
Bai, Juncui
Wei, Junshu
Fu, Shaozu
Liu, Xiangtao
Li, Dan
Zheng, Haixue
author_facet Yang, Wenping
Ru, Yi
Ren, Jingjing
Bai, Juncui
Wei, Junshu
Fu, Shaozu
Liu, Xiangtao
Li, Dan
Zheng, Haixue
author_sort Yang, Wenping
collection PubMed
description Retinoic acid-inducible gene I (RIG-I) is a pattern recognition receptor and is involved in the innate immune response against RNA viruses infection. Here, we demonstrate that the Ras-GTPase-activating protein SH3-domain-binding protein 1 (G3BP1) serves as a positive regulator of the RIG-I-mediated signaling pathway. G3BP1-deficient cells inhibited RNA virus-triggered induction of downstream antiviral genes. Furthermore, we found that G3BP1 inhibited the replication of Sendai virus and vesicular stomatitis virus, indicating a positive regulation of G3BP1 to cellular antiviral responses. Mechanistically, G3BP1 formed a complex with RNF125 and RIG-I, leading to decreased RNF125 via its auto-ubiquitination; thus, promoting expression of RIG-I. Overall, the results suggest a novel mechanism for G3BP1 in the positive regulation of antiviral signaling mediated by RIG-I.
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spelling pubmed-69062972019-12-12 G3BP1 inhibits RNA virus replication by positively regulating RIG-I-mediated cellular antiviral response Yang, Wenping Ru, Yi Ren, Jingjing Bai, Juncui Wei, Junshu Fu, Shaozu Liu, Xiangtao Li, Dan Zheng, Haixue Cell Death Dis Article Retinoic acid-inducible gene I (RIG-I) is a pattern recognition receptor and is involved in the innate immune response against RNA viruses infection. Here, we demonstrate that the Ras-GTPase-activating protein SH3-domain-binding protein 1 (G3BP1) serves as a positive regulator of the RIG-I-mediated signaling pathway. G3BP1-deficient cells inhibited RNA virus-triggered induction of downstream antiviral genes. Furthermore, we found that G3BP1 inhibited the replication of Sendai virus and vesicular stomatitis virus, indicating a positive regulation of G3BP1 to cellular antiviral responses. Mechanistically, G3BP1 formed a complex with RNF125 and RIG-I, leading to decreased RNF125 via its auto-ubiquitination; thus, promoting expression of RIG-I. Overall, the results suggest a novel mechanism for G3BP1 in the positive regulation of antiviral signaling mediated by RIG-I. Nature Publishing Group UK 2019-12-11 /pmc/articles/PMC6906297/ /pubmed/31827077 http://dx.doi.org/10.1038/s41419-019-2178-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yang, Wenping
Ru, Yi
Ren, Jingjing
Bai, Juncui
Wei, Junshu
Fu, Shaozu
Liu, Xiangtao
Li, Dan
Zheng, Haixue
G3BP1 inhibits RNA virus replication by positively regulating RIG-I-mediated cellular antiviral response
title G3BP1 inhibits RNA virus replication by positively regulating RIG-I-mediated cellular antiviral response
title_full G3BP1 inhibits RNA virus replication by positively regulating RIG-I-mediated cellular antiviral response
title_fullStr G3BP1 inhibits RNA virus replication by positively regulating RIG-I-mediated cellular antiviral response
title_full_unstemmed G3BP1 inhibits RNA virus replication by positively regulating RIG-I-mediated cellular antiviral response
title_short G3BP1 inhibits RNA virus replication by positively regulating RIG-I-mediated cellular antiviral response
title_sort g3bp1 inhibits rna virus replication by positively regulating rig-i-mediated cellular antiviral response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906297/
https://www.ncbi.nlm.nih.gov/pubmed/31827077
http://dx.doi.org/10.1038/s41419-019-2178-9
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