A qPCR method for genome editing efficiency determination and single-cell clone screening in human cells

CRISPR/Cas9 technology has been widely used for targeted genome modification both in vivo and in vitro. However, an effective method for evaluating genome editing efficiency and screening single-cell clones for desired modification is still lacking. Here, we developed this real time PCR method based...

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Detalles Bibliográficos
Autores principales: Li, Bo, Ren, Naixia, Yang, Lele, Liu, Junhao, Huang, Qilai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906436/
https://www.ncbi.nlm.nih.gov/pubmed/31827197
http://dx.doi.org/10.1038/s41598-019-55463-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906436/
https://www.ncbi.nlm.nih.gov/pubmed/31827197
http://dx.doi.org/10.1038/s41598-019-55463-6

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