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A qPCR method for genome editing efficiency determination and single-cell clone screening in human cells
CRISPR/Cas9 technology has been widely used for targeted genome modification both in vivo and in vitro. However, an effective method for evaluating genome editing efficiency and screening single-cell clones for desired modification is still lacking. Here, we developed this real time PCR method based...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906436/ https://www.ncbi.nlm.nih.gov/pubmed/31827197 http://dx.doi.org/10.1038/s41598-019-55463-6 |