Extracellular vesicles from symbiotic vaginal lactobacilli inhibit HIV-1 infection of human tissues

The vaginal microbiota, dominated by Lactobacillus spp., plays a key role in preventing HIV-1 transmission. Here, we investigate whether the anti-HIV effect of lactobacilli is mediated by extracellular vesicles (EVs) released by these bacteria. Human cervico-vaginal and tonsillar tissues ex vivo, an...

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Autores principales: Ñahui Palomino, Rogers A., Vanpouille, Christophe, Laghi, Luca, Parolin, Carola, Melikov, Kamran, Backlund, Peter, Vitali, Beatrice, Margolis, Leonid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906448/
https://www.ncbi.nlm.nih.gov/pubmed/31827089
http://dx.doi.org/10.1038/s41467-019-13468-9
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author Ñahui Palomino, Rogers A.
Vanpouille, Christophe
Laghi, Luca
Parolin, Carola
Melikov, Kamran
Backlund, Peter
Vitali, Beatrice
Margolis, Leonid
author_facet Ñahui Palomino, Rogers A.
Vanpouille, Christophe
Laghi, Luca
Parolin, Carola
Melikov, Kamran
Backlund, Peter
Vitali, Beatrice
Margolis, Leonid
author_sort Ñahui Palomino, Rogers A.
collection PubMed
description The vaginal microbiota, dominated by Lactobacillus spp., plays a key role in preventing HIV-1 transmission. Here, we investigate whether the anti-HIV effect of lactobacilli is mediated by extracellular vesicles (EVs) released by these bacteria. Human cervico-vaginal and tonsillar tissues ex vivo, and cell lines were infected with HIV-1 and treated with EVs released by lactobacilli isolated from vaginas of healthy women. EVs released by L. crispatus BC3 and L. gasseri BC12 protect tissues ex vivo and isolated cells from HIV-1 infection. This protection is associated with a decrease of viral attachment to target cells and viral entry due to diminished exposure of Env that mediates virus-cell interactions. Inhibition of HIV-1 infection is associated with the presence in EVs of several proteins and metabolites. Our findings demonstrate that the protective effect of Lactobacillus against HIV-1 is, in part, mediated by EVs released by these symbiotic bacteria. If confirmed in vivo, this finding may lead to new strategies to prevent male-to-female sexual HIV-1 transmission.
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spelling pubmed-69064482019-12-13 Extracellular vesicles from symbiotic vaginal lactobacilli inhibit HIV-1 infection of human tissues Ñahui Palomino, Rogers A. Vanpouille, Christophe Laghi, Luca Parolin, Carola Melikov, Kamran Backlund, Peter Vitali, Beatrice Margolis, Leonid Nat Commun Article The vaginal microbiota, dominated by Lactobacillus spp., plays a key role in preventing HIV-1 transmission. Here, we investigate whether the anti-HIV effect of lactobacilli is mediated by extracellular vesicles (EVs) released by these bacteria. Human cervico-vaginal and tonsillar tissues ex vivo, and cell lines were infected with HIV-1 and treated with EVs released by lactobacilli isolated from vaginas of healthy women. EVs released by L. crispatus BC3 and L. gasseri BC12 protect tissues ex vivo and isolated cells from HIV-1 infection. This protection is associated with a decrease of viral attachment to target cells and viral entry due to diminished exposure of Env that mediates virus-cell interactions. Inhibition of HIV-1 infection is associated with the presence in EVs of several proteins and metabolites. Our findings demonstrate that the protective effect of Lactobacillus against HIV-1 is, in part, mediated by EVs released by these symbiotic bacteria. If confirmed in vivo, this finding may lead to new strategies to prevent male-to-female sexual HIV-1 transmission. Nature Publishing Group UK 2019-12-11 /pmc/articles/PMC6906448/ /pubmed/31827089 http://dx.doi.org/10.1038/s41467-019-13468-9 Text en © This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ñahui Palomino, Rogers A.
Vanpouille, Christophe
Laghi, Luca
Parolin, Carola
Melikov, Kamran
Backlund, Peter
Vitali, Beatrice
Margolis, Leonid
Extracellular vesicles from symbiotic vaginal lactobacilli inhibit HIV-1 infection of human tissues
title Extracellular vesicles from symbiotic vaginal lactobacilli inhibit HIV-1 infection of human tissues
title_full Extracellular vesicles from symbiotic vaginal lactobacilli inhibit HIV-1 infection of human tissues
title_fullStr Extracellular vesicles from symbiotic vaginal lactobacilli inhibit HIV-1 infection of human tissues
title_full_unstemmed Extracellular vesicles from symbiotic vaginal lactobacilli inhibit HIV-1 infection of human tissues
title_short Extracellular vesicles from symbiotic vaginal lactobacilli inhibit HIV-1 infection of human tissues
title_sort extracellular vesicles from symbiotic vaginal lactobacilli inhibit hiv-1 infection of human tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906448/
https://www.ncbi.nlm.nih.gov/pubmed/31827089
http://dx.doi.org/10.1038/s41467-019-13468-9
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