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Steady expression of high oleic acid in peanut bred by marker-assisted backcrossing for fatty acid desaturase mutant alleles and its effect on seed germination along with other seedling traits

Peanut (Arachis hypogaea L.) is an important nutrient-rich food legume and valued for its good quality cooking oil. The fatty acid content is the major determinant of the quality of the edible oil. The oils containing higher monounsaturated fatty acid are preferred for improved shelf life and potent...

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Autores principales: Bera, Sandip K., Kamdar, Jignesh H., Kasundra, Swati V., Patel, Sahil V., Jasani, Mital D., Maurya, A. K., Dash, P., Chandrashekar, Ajay B., Rani, Kirti, Manivannan, N., Janila, Pasupuleti, Pandey, Manish K., Vasanthi, R. P., Dobariya, K. L., Radhakrishnan, T., Varshney, Rajeev K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6910123/
https://www.ncbi.nlm.nih.gov/pubmed/31830093
http://dx.doi.org/10.1371/journal.pone.0226252
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author Bera, Sandip K.
Kamdar, Jignesh H.
Kasundra, Swati V.
Patel, Sahil V.
Jasani, Mital D.
Maurya, A. K.
Dash, P.
Chandrashekar, Ajay B.
Rani, Kirti
Manivannan, N.
Janila, Pasupuleti
Pandey, Manish K.
Vasanthi, R. P.
Dobariya, K. L.
Radhakrishnan, T.
Varshney, Rajeev K.
author_facet Bera, Sandip K.
Kamdar, Jignesh H.
Kasundra, Swati V.
Patel, Sahil V.
Jasani, Mital D.
Maurya, A. K.
Dash, P.
Chandrashekar, Ajay B.
Rani, Kirti
Manivannan, N.
Janila, Pasupuleti
Pandey, Manish K.
Vasanthi, R. P.
Dobariya, K. L.
Radhakrishnan, T.
Varshney, Rajeev K.
author_sort Bera, Sandip K.
collection PubMed
description Peanut (Arachis hypogaea L.) is an important nutrient-rich food legume and valued for its good quality cooking oil. The fatty acid content is the major determinant of the quality of the edible oil. The oils containing higher monounsaturated fatty acid are preferred for improved shelf life and potential health benefits. Therefore, a high oleic/linoleic fatty acid ratio is the target trait in an advanced breeding program. The two mutant alleles, ahFAD2A (on linkage group a09) and ahFAD2B (on linkage group b09) control fatty acid composition for higher oleic/linoleic ratio in peanut. In the present study, marker-assisted backcrossing was employed for the introgression of two FAD2 mutant alleles from SunOleic95R into the chromosome of ICGV06100, a high oil content peanut breeding line. In the marker-assisted backcrossing-introgression lines, a 97% increase in oleic acid, and a 92% reduction in linoleic acid content was observed in comparison to the recurrent parent. Besides, the oleic/linoleic ratio was increased to 25 with respect to the recurrent parent, which was only 1.2. The most significant outcome was the stable expression of oil-content, oleic acid, linoleic acid, and palmitic acid in the marker-assisted backcrossing-introgression lines over the locations. No significant difference was observed between high oleic and normal oleic in peanuts for seedling traits except germination percentage. In addition, marker-assisted backcrossing-introgression lines exhibited higher yield and resistance to foliar fungal diseases, i.e., late leaf spot and rust.
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spelling pubmed-69101232019-12-27 Steady expression of high oleic acid in peanut bred by marker-assisted backcrossing for fatty acid desaturase mutant alleles and its effect on seed germination along with other seedling traits Bera, Sandip K. Kamdar, Jignesh H. Kasundra, Swati V. Patel, Sahil V. Jasani, Mital D. Maurya, A. K. Dash, P. Chandrashekar, Ajay B. Rani, Kirti Manivannan, N. Janila, Pasupuleti Pandey, Manish K. Vasanthi, R. P. Dobariya, K. L. Radhakrishnan, T. Varshney, Rajeev K. PLoS One Research Article Peanut (Arachis hypogaea L.) is an important nutrient-rich food legume and valued for its good quality cooking oil. The fatty acid content is the major determinant of the quality of the edible oil. The oils containing higher monounsaturated fatty acid are preferred for improved shelf life and potential health benefits. Therefore, a high oleic/linoleic fatty acid ratio is the target trait in an advanced breeding program. The two mutant alleles, ahFAD2A (on linkage group a09) and ahFAD2B (on linkage group b09) control fatty acid composition for higher oleic/linoleic ratio in peanut. In the present study, marker-assisted backcrossing was employed for the introgression of two FAD2 mutant alleles from SunOleic95R into the chromosome of ICGV06100, a high oil content peanut breeding line. In the marker-assisted backcrossing-introgression lines, a 97% increase in oleic acid, and a 92% reduction in linoleic acid content was observed in comparison to the recurrent parent. Besides, the oleic/linoleic ratio was increased to 25 with respect to the recurrent parent, which was only 1.2. The most significant outcome was the stable expression of oil-content, oleic acid, linoleic acid, and palmitic acid in the marker-assisted backcrossing-introgression lines over the locations. No significant difference was observed between high oleic and normal oleic in peanuts for seedling traits except germination percentage. In addition, marker-assisted backcrossing-introgression lines exhibited higher yield and resistance to foliar fungal diseases, i.e., late leaf spot and rust. Public Library of Science 2019-12-12 /pmc/articles/PMC6910123/ /pubmed/31830093 http://dx.doi.org/10.1371/journal.pone.0226252 Text en © 2019 Bera et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Bera, Sandip K.
Kamdar, Jignesh H.
Kasundra, Swati V.
Patel, Sahil V.
Jasani, Mital D.
Maurya, A. K.
Dash, P.
Chandrashekar, Ajay B.
Rani, Kirti
Manivannan, N.
Janila, Pasupuleti
Pandey, Manish K.
Vasanthi, R. P.
Dobariya, K. L.
Radhakrishnan, T.
Varshney, Rajeev K.
Steady expression of high oleic acid in peanut bred by marker-assisted backcrossing for fatty acid desaturase mutant alleles and its effect on seed germination along with other seedling traits
title Steady expression of high oleic acid in peanut bred by marker-assisted backcrossing for fatty acid desaturase mutant alleles and its effect on seed germination along with other seedling traits
title_full Steady expression of high oleic acid in peanut bred by marker-assisted backcrossing for fatty acid desaturase mutant alleles and its effect on seed germination along with other seedling traits
title_fullStr Steady expression of high oleic acid in peanut bred by marker-assisted backcrossing for fatty acid desaturase mutant alleles and its effect on seed germination along with other seedling traits
title_full_unstemmed Steady expression of high oleic acid in peanut bred by marker-assisted backcrossing for fatty acid desaturase mutant alleles and its effect on seed germination along with other seedling traits
title_short Steady expression of high oleic acid in peanut bred by marker-assisted backcrossing for fatty acid desaturase mutant alleles and its effect on seed germination along with other seedling traits
title_sort steady expression of high oleic acid in peanut bred by marker-assisted backcrossing for fatty acid desaturase mutant alleles and its effect on seed germination along with other seedling traits
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6910123/
https://www.ncbi.nlm.nih.gov/pubmed/31830093
http://dx.doi.org/10.1371/journal.pone.0226252
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