In situ Microfluidic Cryofixation for Cryo Focused Ion Beam Milling and Cryo Electron Tomography

We present a microfluidic platform for studying structure-function relationships at the cellular level by connecting video rate live cell imaging with in situ microfluidic cryofixation and cryo-electron tomography of near natively preserved, unstained specimens. Correlative light and electron micros...

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Detalles Bibliográficos
Autores principales: Fuest, Marie, Schaffer, Miroslava, Nocera, Giovanni Marco, Galilea-Kleinsteuber, Rodrigo I., Messling, Jan-Erik, Heymann, Michael, Plitzko, Jürgen M., Burg, Thomas P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911106/
https://www.ncbi.nlm.nih.gov/pubmed/31836773
http://dx.doi.org/10.1038/s41598-019-55413-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911106/
https://www.ncbi.nlm.nih.gov/pubmed/31836773
http://dx.doi.org/10.1038/s41598-019-55413-2

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