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In situ Microfluidic Cryofixation for Cryo Focused Ion Beam Milling and Cryo Electron Tomography
We present a microfluidic platform for studying structure-function relationships at the cellular level by connecting video rate live cell imaging with in situ microfluidic cryofixation and cryo-electron tomography of near natively preserved, unstained specimens. Correlative light and electron micros...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911106/ https://www.ncbi.nlm.nih.gov/pubmed/31836773 http://dx.doi.org/10.1038/s41598-019-55413-2 |