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Nutrient germination improves DNA recovery from industrial Bacillus subtilis endospores during qPCR enumeration assays

Growth-independent microbial enumeration methods such as quantitative PCR require the efficient extraction of genomic DNA from targeted cells. Bacillus endospores are popular inclusions in commercial products due to their hardiness and metabolic dormancy; however, this hardiness is known to render B...

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Detalles Bibliográficos
Autores principales: Gorsuch, John P., Woodruff, Peyton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911885/
https://www.ncbi.nlm.nih.gov/pubmed/31872115
http://dx.doi.org/10.1016/j.heliyon.2019.e02917