Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes

While Tn-Seq is a powerful tool to determine genome-wide bacterial fitness in high-throughput, culturing transposon-mutant libraries in pools can mask community or other complex single-cell phenotypes. Droplet Tn-Seq (dTn-Seq) solves this problem by microfluidics facilitated encapsulation of individ...

Descripción completa

Detalles Bibliográficos
Autores principales: Thibault, Derek, Jensen, Paul A., Wood, Stephen, Qabar, Christine, Clark, Stacie, Shainheit, Mara G., Isberg, Ralph R., van Opijnen, Tim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6914776/
https://www.ncbi.nlm.nih.gov/pubmed/31844066
http://dx.doi.org/10.1038/s41467-019-13719-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6914776/
https://www.ncbi.nlm.nih.gov/pubmed/31844066
http://dx.doi.org/10.1038/s41467-019-13719-9

Ejemplares similares