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Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes

While Tn-Seq is a powerful tool to determine genome-wide bacterial fitness in high-throughput, culturing transposon-mutant libraries in pools can mask community or other complex single-cell phenotypes. Droplet Tn-Seq (dTn-Seq) solves this problem by microfluidics facilitated encapsulation of individ...

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Detalles Bibliográficos
Autores principales:	Thibault, Derek, Jensen, Paul A., Wood, Stephen, Qabar, Christine, Clark, Stacie, Shainheit, Mara G., Isberg, Ralph R., van Opijnen, Tim
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6914776/ https://www.ncbi.nlm.nih.gov/pubmed/31844066 http://dx.doi.org/10.1038/s41467-019-13719-9

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