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Fast and Reliable PCR Amplification from Aspergillus fumigatus Spore Suspension Without Traditional DNA Extraction

Aspergillus fumigatus is an opportunistic human pathogenic mold. DNA extraction from this fungus is usually performed by mechanical perturbation of cells, as it possesses a rigid and complex cell wall. While this is not problematic for single isolates, it can be time consuming for large numbers of s...

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Detalles Bibliográficos
Autores principales: Fraczek, Marcin G., Zhao, Can, Dineen, Lauren, Lebedinec, Ressa, Bowyer, Paul, Bromley, Michael, Delneri, Daniela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6916316/
https://www.ncbi.nlm.nih.gov/pubmed/31518062
http://dx.doi.org/10.1002/cpmc.89