Real-time PCR for direct aptamer quantification on functionalized graphene surfaces

In this study, we develop a real-time PCR strategy to directly detect and quantify DNA aptamers on functionalized graphene surfaces using a Staphylococcus aureus aptamer (SA20) as demonstration case. We show that real-time PCR allowed aptamer quantification in the range of 0.05 fg to 2.5 ng. Using t...

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Detalles Bibliográficos
Autores principales: dos Santos, Viviane C. F., Almeida, Nathalie B. F., de Sousa, Thiago A. S. L., Araujo, Eduardo N. D., de Andrade, Antero S. R., Plentz, Flávio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6917711/
https://www.ncbi.nlm.nih.gov/pubmed/31848419
http://dx.doi.org/10.1038/s41598-019-55892-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6917711/
https://www.ncbi.nlm.nih.gov/pubmed/31848419
http://dx.doi.org/10.1038/s41598-019-55892-3

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