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PRIMEval: Optimization and screening of multiplex oligonucleotide assays
The development of multiplex polymerase chain reaction and microarray assays is challenging due to primer dimer formation, unspecific hybridization events, the generation of unspecific by-products, primer depletion, and thus lower amplification efficiencies. We have developed a software workflow wit...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6917790/ https://www.ncbi.nlm.nih.gov/pubmed/31848453 http://dx.doi.org/10.1038/s41598-019-55883-4 |