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Supercritical fluid extraction of β-carotene from ripe bitter melon pericarp

Study ascertained the recovery of β-carotene from enzyme-treated (enzyme load of 167 U/g) pericarp of ripe bitter melon using supercritical fluid extraction (SFE) technique. Effect of different pressure (ranged from 150–450 bar), carbon dioxide (CO(2)) flow rates (ranged from 15 to 55 ml/min), tempe...

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Autores principales: Patel, Avinash Singh, Kar, Abhijit, Dash, Sukanta, Dash, Sanjaya K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6917792/
https://www.ncbi.nlm.nih.gov/pubmed/31848376
http://dx.doi.org/10.1038/s41598-019-55481-4
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author Patel, Avinash Singh
Kar, Abhijit
Dash, Sukanta
Dash, Sanjaya K.
author_facet Patel, Avinash Singh
Kar, Abhijit
Dash, Sukanta
Dash, Sanjaya K.
author_sort Patel, Avinash Singh
collection PubMed
description Study ascertained the recovery of β-carotene from enzyme-treated (enzyme load of 167 U/g) pericarp of ripe bitter melon using supercritical fluid extraction (SFE) technique. Effect of different pressure (ranged from 150–450 bar), carbon dioxide (CO(2)) flow rates (ranged from 15 to 55 ml/min), temperatures (from 50 to 90 °C), and extraction periods (from 45–225 minutes) were observed on the extraction efficiency of β-carotene. Results showed that extraction pressure (X(1)) among extraction parameters had the most significant (p < 0.05) effect on extraction efficiency of the β-carotene followed by allowed extraction time (X(4)), CO(2) flow rate (X(2)) and the temperature of the extraction (X(3)). The maximum yield of 90.12% of β-carotene from lyophilized enzymatic pretreated ripe bitter melon pericarp was achieved at the pressure of approx. 390 bar, flow rate of 35 mL/min, temperature at 70 °C and extraction time of 190 min, respectively. Based on the accelerated storage study the 70% retention shelf life of the β-carotene into extract was estimated up to 2.27 months at 10 °C and up to 3.21 months at 5 °C.
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spelling pubmed-69177922019-12-19 Supercritical fluid extraction of β-carotene from ripe bitter melon pericarp Patel, Avinash Singh Kar, Abhijit Dash, Sukanta Dash, Sanjaya K. Sci Rep Article Study ascertained the recovery of β-carotene from enzyme-treated (enzyme load of 167 U/g) pericarp of ripe bitter melon using supercritical fluid extraction (SFE) technique. Effect of different pressure (ranged from 150–450 bar), carbon dioxide (CO(2)) flow rates (ranged from 15 to 55 ml/min), temperatures (from 50 to 90 °C), and extraction periods (from 45–225 minutes) were observed on the extraction efficiency of β-carotene. Results showed that extraction pressure (X(1)) among extraction parameters had the most significant (p < 0.05) effect on extraction efficiency of the β-carotene followed by allowed extraction time (X(4)), CO(2) flow rate (X(2)) and the temperature of the extraction (X(3)). The maximum yield of 90.12% of β-carotene from lyophilized enzymatic pretreated ripe bitter melon pericarp was achieved at the pressure of approx. 390 bar, flow rate of 35 mL/min, temperature at 70 °C and extraction time of 190 min, respectively. Based on the accelerated storage study the 70% retention shelf life of the β-carotene into extract was estimated up to 2.27 months at 10 °C and up to 3.21 months at 5 °C. Nature Publishing Group UK 2019-12-17 /pmc/articles/PMC6917792/ /pubmed/31848376 http://dx.doi.org/10.1038/s41598-019-55481-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Patel, Avinash Singh
Kar, Abhijit
Dash, Sukanta
Dash, Sanjaya K.
Supercritical fluid extraction of β-carotene from ripe bitter melon pericarp
title Supercritical fluid extraction of β-carotene from ripe bitter melon pericarp
title_full Supercritical fluid extraction of β-carotene from ripe bitter melon pericarp
title_fullStr Supercritical fluid extraction of β-carotene from ripe bitter melon pericarp
title_full_unstemmed Supercritical fluid extraction of β-carotene from ripe bitter melon pericarp
title_short Supercritical fluid extraction of β-carotene from ripe bitter melon pericarp
title_sort supercritical fluid extraction of β-carotene from ripe bitter melon pericarp
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6917792/
https://www.ncbi.nlm.nih.gov/pubmed/31848376
http://dx.doi.org/10.1038/s41598-019-55481-4
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