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Genetic and metabolic effects of ripening mutations and vine detachment on tomato fruit quality
Tomato (Solanum lycopersicum) fruit ripening is regulated co‐operatively by the action of ethylene and a hierarchy of transcription factors, including RIPENING INHIBITOR (RIN) and NON‐RIPENING (NOR). Mutations in these two genes have been adopted commercially to delay ripening, and accompanying text...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920187/ https://www.ncbi.nlm.nih.gov/pubmed/31131540 http://dx.doi.org/10.1111/pbi.13176 |
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author | Osorio, Sonia Carneiro, Raphael T. Lytovchenko, Anna McQuinn, Ryan Sørensen, Iben Vallarino, José G. Giovannoni, James J. Fernie, Alisdair R. Rose, Jocelyn K. C. |
author_facet | Osorio, Sonia Carneiro, Raphael T. Lytovchenko, Anna McQuinn, Ryan Sørensen, Iben Vallarino, José G. Giovannoni, James J. Fernie, Alisdair R. Rose, Jocelyn K. C. |
author_sort | Osorio, Sonia |
collection | PubMed |
description | Tomato (Solanum lycopersicum) fruit ripening is regulated co‐operatively by the action of ethylene and a hierarchy of transcription factors, including RIPENING INHIBITOR (RIN) and NON‐RIPENING (NOR). Mutations in these two genes have been adopted commercially to delay ripening, and accompanying textural deterioration, as a means to prolong shelf life. However, these mutations also affect desirable traits associated with colour and nutritional value, although the extent of this trade‐off has not been assessed in detail. Here, we evaluated changes in tomato fruit pericarp primary metabolite and carotenoid pigment profiles, as well as the dynamics of specific associated transcripts, in the rin and nor mutants during late development and postharvest storage, as well of those of the partially ripening delayed fruit ripening (dfd) tomato genotype. These profiles were compared with those of the wild‐type tomato cultivars Ailsa Craig (AC) and M82. We also evaluated the metabolic composition of M82 fruit ripened on or off the vine over a similar period. In general, the dfd mutation resulted in prolonged firmness and maintenance of quality traits without compromising key metabolites (sucrose, glucose/fructose and glucose) and sectors of intermediary metabolism, including tricarboxylic acid cycle intermediates. Our analysis also provided insights into the regulation of carotenoid formation and highlighted the importance of the polyamine, putrescine, in extending fruit shelf life. Finally, the metabolic composition analysis of M82 fruit ripened on or off the vine provided insights into the import into fruit of compounds, such as sucrose, during ripening. |
format | Online Article Text |
id | pubmed-6920187 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69201872019-12-27 Genetic and metabolic effects of ripening mutations and vine detachment on tomato fruit quality Osorio, Sonia Carneiro, Raphael T. Lytovchenko, Anna McQuinn, Ryan Sørensen, Iben Vallarino, José G. Giovannoni, James J. Fernie, Alisdair R. Rose, Jocelyn K. C. Plant Biotechnol J Research Articles Tomato (Solanum lycopersicum) fruit ripening is regulated co‐operatively by the action of ethylene and a hierarchy of transcription factors, including RIPENING INHIBITOR (RIN) and NON‐RIPENING (NOR). Mutations in these two genes have been adopted commercially to delay ripening, and accompanying textural deterioration, as a means to prolong shelf life. However, these mutations also affect desirable traits associated with colour and nutritional value, although the extent of this trade‐off has not been assessed in detail. Here, we evaluated changes in tomato fruit pericarp primary metabolite and carotenoid pigment profiles, as well as the dynamics of specific associated transcripts, in the rin and nor mutants during late development and postharvest storage, as well of those of the partially ripening delayed fruit ripening (dfd) tomato genotype. These profiles were compared with those of the wild‐type tomato cultivars Ailsa Craig (AC) and M82. We also evaluated the metabolic composition of M82 fruit ripened on or off the vine over a similar period. In general, the dfd mutation resulted in prolonged firmness and maintenance of quality traits without compromising key metabolites (sucrose, glucose/fructose and glucose) and sectors of intermediary metabolism, including tricarboxylic acid cycle intermediates. Our analysis also provided insights into the regulation of carotenoid formation and highlighted the importance of the polyamine, putrescine, in extending fruit shelf life. Finally, the metabolic composition analysis of M82 fruit ripened on or off the vine provided insights into the import into fruit of compounds, such as sucrose, during ripening. John Wiley and Sons Inc. 2019-06-11 2020-01 /pmc/articles/PMC6920187/ /pubmed/31131540 http://dx.doi.org/10.1111/pbi.13176 Text en © 2019 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Osorio, Sonia Carneiro, Raphael T. Lytovchenko, Anna McQuinn, Ryan Sørensen, Iben Vallarino, José G. Giovannoni, James J. Fernie, Alisdair R. Rose, Jocelyn K. C. Genetic and metabolic effects of ripening mutations and vine detachment on tomato fruit quality |
title | Genetic and metabolic effects of ripening mutations and vine detachment on tomato fruit quality |
title_full | Genetic and metabolic effects of ripening mutations and vine detachment on tomato fruit quality |
title_fullStr | Genetic and metabolic effects of ripening mutations and vine detachment on tomato fruit quality |
title_full_unstemmed | Genetic and metabolic effects of ripening mutations and vine detachment on tomato fruit quality |
title_short | Genetic and metabolic effects of ripening mutations and vine detachment on tomato fruit quality |
title_sort | genetic and metabolic effects of ripening mutations and vine detachment on tomato fruit quality |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920187/ https://www.ncbi.nlm.nih.gov/pubmed/31131540 http://dx.doi.org/10.1111/pbi.13176 |
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