Computational approaches for effective CRISPR guide RNA design and evaluation

The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/ CRISPR-associated (Cas) system has emerged as the main technology for gene editing. Successful editing by CRISPR requires an appropriate Cas protein and guide RNA. However, low cleavage efficiency and off-target effects hamper th...

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Detalles Bibliográficos
Autores principales: Liu, Guanqing, Zhang, Yong, Zhang, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Network of Computational and Structural Biotechnology 2019
Materias:
Review Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6921152/
https://www.ncbi.nlm.nih.gov/pubmed/31890142
http://dx.doi.org/10.1016/j.csbj.2019.11.006

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6921152/
https://www.ncbi.nlm.nih.gov/pubmed/31890142
http://dx.doi.org/10.1016/j.csbj.2019.11.006

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