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Image-based analysis of living mammalian cells using label-free 3D refractive index maps reveals new organelle dynamics and dry mass flux

Holo-tomographic microscopy (HTM) is a label-free microscopy method reporting the fine changes of a cell’s refractive indices (RIs) in three dimensions at high spatial and temporal resolution. By combining HTM with epifluorescence, we demonstrate that mammalian cellular organelles such as lipid drop...

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Detalles Bibliográficos
Autores principales: Sandoz, Patrick A., Tremblay, Christopher, van der Goot, F. Gisou, Frechin, Mathieu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6922317/
https://www.ncbi.nlm.nih.gov/pubmed/31856161
http://dx.doi.org/10.1371/journal.pbio.3000553