Cargando…

Image-based analysis of living mammalian cells using label-free 3D refractive index maps reveals new organelle dynamics and dry mass flux

Holo-tomographic microscopy (HTM) is a label-free microscopy method reporting the fine changes of a cell’s refractive indices (RIs) in three dimensions at high spatial and temporal resolution. By combining HTM with epifluorescence, we demonstrate that mammalian cellular organelles such as lipid drop...

Descripción completa

Detalles Bibliográficos
Autores principales:	Sandoz, Patrick A., Tremblay, Christopher, van der Goot, F. Gisou, Frechin, Mathieu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2019
Materias:	Methods and Resources
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6922317/ https://www.ncbi.nlm.nih.gov/pubmed/31856161 http://dx.doi.org/10.1371/journal.pbio.3000553

Ejemplares similares

High sensitivity and low-cost flavin luciferase (FLUX(Vc))-based reporter gene for mammalian cell expression
por: Phonbuppha, Jittima, et al.
Publicado: (2023)

Reversible association with motor proteins (RAMP): A streptavidin-based method to manipulate organelle positioning
por: Guardia, Carlos M., et al.
Publicado: (2019)

In situ artificial contact sites (ISACS) between synthetic and endogenous organelle membranes allow for quantification of protein-tethering activities
por: Milanini, Julie, et al.
Publicado: (2022)

Multisite imaging of neural activity using a genetically encoded calcium sensor in the honey bee
por: Carcaud, Julie, et al.
Publicado: (2023)

Mammalian lectin arrays for screening host–microbe interactions
por: Jégouzo, Sabine A. F., et al.
Publicado: (2020)

A pathogen-specific isotope tracing approach reveals metabolic activities and fluxes of intracellular Salmonella
por: Mitosch, Karin, et al.
Publicado: (2023)

Multivariate phenotype analysis enables genome-wide inference of mammalian gene function
por: Nicholson, George, et al.
Publicado: (2022)

Development of a novel mammalian display system for selection of antibodies against membrane proteins
por: Robertson, Nathan, et al.
Publicado: (2021)

Proteomics of a fuzzy organelle: interphase chromatin
por: Kustatscher, Georg, et al.
Publicado: (2014)

Metabolic turnover and dynamics of modified ribonucleosides by (13)C labeling
por: Gameiro, Paulo A., et al.
Publicado: (2021)

Cell type–specific labeling of newly synthesized proteins by puromycin inactivation
por: Cabrera-Cabrera, Florencia, et al.
Publicado: (2023)

Ultrahigh resolution MS(1)/MS(2)-based reconstruction of metabolic networks in mammalian cells reveals changes for selenite and arsenite action
por: Fan, Teresa W.-M., et al.
Publicado: (2022)

Impact of inherent biases built into proteomic techniques: Proximity labeling and affinity capture compared
por: Moreira, Claudia Maria do Nascimento, et al.
Publicado: (2022)

Measurement of gluconeogenesis by (2)H(2)O labeling and mass isotopomer distribution analysis
por: Ziari, Naveed, et al.
Publicado: (2023)

Accessing isotopically labeled proteins containing genetically encoded phosphoserine for NMR with optimized expression conditions
por: Vesely, Cat Hoang, et al.
Publicado: (2022)

Depletion of endogenously biotinylated carboxylases enhances the sensitivity of TurboID-mediated proximity labeling in Caenorhabditis elegans
por: Artan, Murat, et al.
Publicado: (2022)

Construction and validation of customized genomes for human and mouse ribosomal DNA mapping
por: George, Subin S., et al.
Publicado: (2023)

A Comprehensive Comparison of Transmembrane Domains Reveals Organelle-Specific Properties
por: Sharpe, Hayley J., et al.
Publicado: (2010)

Precise genomic mapping of 5-hydroxymethylcytosine via covalent tether-directed sequencing
por: Gibas, Povilas, et al.
Publicado: (2020)

Systematic and scalable genome-wide essentiality mapping to identify nonessential genes in phages
por: Piya, Denish, et al.
Publicado: (2023)

Evaluation of RNAi and CRISPR technologies by large-scale gene expression profiling in the Connectivity Map
por: Smith, Ian, et al.
Publicado: (2017)

A map of protein dynamics during cell-cycle progression and cell-cycle exit
por: Gookin, Sara, et al.
Publicado: (2017)

High-resolution 3D imaging and topological mapping of the lymph node conduit system
por: Kelch, Inken D., et al.
Publicado: (2019)

SCRINSHOT enables spatial mapping of cell states in tissue sections with single-cell resolution
por: Sountoulidis, Alexandros, et al.
Publicado: (2020)

Performing group-level functional image analyses based on homologous functional regions mapped in individuals
por: Li, Meiling, et al.
Publicado: (2019)

Single-cell mapping reveals new markers and functions of lymphatic endothelial cells in lymph nodes
por: Fujimoto, Noriki, et al.
Publicado: (2020)

High-throughput characterization of photocrosslinker-bearing ion channel variants to map residues critical for function and pharmacology
por: Braun, Nina, et al.
Publicado: (2021)

A new technique for genome-wide mapping of nucleotide excision repair without immunopurification of damaged DNA
por: Wu, Sizhong, et al.
Publicado: (2022)

Cellular labeling of endogenous retrovirus replication (CLEVR) reveals de novo insertions of the gypsy retrotransposable element in cell culture and in both neurons and glial cells of aging fruit flies
por: Chang, Yung-Heng, et al.
Publicado: (2019)

Mapping the MOB proteins’ proximity network reveals a unique interaction between human MOB3C and the RNase P complex
por: Elkholi, Islam E., et al.
Publicado: (2023)

A recombinant technique for mapping functional sites of heterotrimeric collagen helices: Collagen IV CB3 fragment as a prototype for integrin binding
por: Boudko, Sergei P., et al.
Publicado: (2023)

Mitochondria transplantation between living cells
por: Gäbelein, Christoph G., et al.
Publicado: (2022)

Direct visualization of single-molecule membrane protein interactions in living cells
por: Kim, Do-Hyeon, et al.
Publicado: (2018)

Proteome-wide analysis of chaperone-mediated autophagy targeting motifs
por: Kirchner, Philipp, et al.
Publicado: (2019)

Multiple marker abundance profiling: combining selected reaction monitoring and data‐dependent acquisition for rapid estimation of organelle abundance in subcellular samples
por: Hooper, Cornelia M., et al.
Publicado: (2017)

Whole-cell FRET monitoring of transcription factor activities enables functional annotation of signal transduction systems in living bacteria
por: Wang, Pengchao, et al.
Publicado: (2022)

A light-inducible protein clustering system for in vivo analysis of α-synuclein aggregation in Parkinson disease
por: Bérard, Morgan, et al.
Publicado: (2022)

LIVECell—A large-scale dataset for label-free live cell segmentation
por: Edlund, Christoffer, et al.
Publicado: (2021)

Reporters to mark and eliminate basal or luminal epithelial cells in culture and in vivo
por: Sonzogni, Olmo, et al.
Publicado: (2018)

Universal DNA methylation age across mammalian tissues
por: Lu, A. T., et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_rkg1ogmd0areuhspu1lc8m464b