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Labeling surface proteins with high specificity: Intrinsic limitations of phosphopantetheinyl transferase systems
OBJECTIVE: Fluorescent labeling of specific cell-surface proteins enables a manifold of techniques to study their function in health and disease. A frequently cited family of methods employs phosphopantetheinyl transferases (PPTases) to attach probes, provided as conjugates of Coenzyme A. This metho...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6922365/ https://www.ncbi.nlm.nih.gov/pubmed/31856184 http://dx.doi.org/10.1371/journal.pone.0226579 |